Restriction map for
pFN21AE5476
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 438 CCGC CCGC(-3/-1)
AcsI 106 GAATTT [GA]^AATT[CT]
AflIII 647 ACACGT A^C[GA][CT]GT
ApaI 583 GGGCCC GGGCC^C
ApoI 106 GAATTT [GA]^AATT[CT]
AspHI 383 GAGCTC G[AT]GC[AT]^C
BclI 593 TGATCA T^GATCA
BlpI 370 GCTCAGC GC^T[ACGT]AGC
BpmI 225 CTCCAG CTGGAG(16/14)
Bpu1102I 370 GCTCAGC GC^T[ACGT]AGC
BsaAI 648 CACGTA [CT]AC^GT[GA]
BsaJI 71 CCTTGG C^C[ACGT][ACGT]GG
BseRI 553 CTCCTC GAGGAG(10/8)
BsiHKAI 383 GAGCTC G[AT]GC[AT]^C
BsmFI 753 GTCCC GGGAC(10/14)
BspHI 479 TCATGA T^CATGA
BspMI 54 ACCTGC ACCTGC(4/8)
BsrDI 318 CATTGC GCAATG(2/0)
CelII 370 GCTCAGC GC^T[ACGT]AGC
DraII 751 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 949 GAAGAG CTCTTC(1/4)
EcoO109I 751 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 71 CCTTGG C^C[AT][AT]GG
FbaI 593 TGATCA T^GATCA
HphI 221 TCACC GGTGA(8/7)
Ksp632I 949 GAAGAG CTCTTC(1/4)
MaeII 649 ACGT A^CGT
MseI 688 TTAA T^TAA
NspI 200 ACATGC [GA]CATG^[CT]
PleI 325 GACTC GAGTC(4/5)
PpuMI 751 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 479 TCATGA T^CATGA
SacI 383 GAGCTC GAGCT^C
SfcI 872 CTATAG C^T[GA][CT]AG
SmlI 747 CTTGAG C^T[CT][GA]AG
StyI 71 CCTTGG C^C[AT][AT]GG
Tru9I 688 TTAA T^TAA
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 561 GGTCC G^G[AT]CC
752 GGTCC
BalI 263 TGGCCA TGG^CCA
446 TGGCCA
BbvI 362 GCTGC GCAGC(8/12)
718 GCTGC
BsmI 58 GCATTC GAATGC(1/-1)
537 GCATTC
BstF5I 735 CATCC GGATG(2/0)
853 CATCC
EaeI 263 TGGCCA [CT]^GGCC[GA]
446 TGGCCA
Fnu4HI 362 GCTGC GC^[ACGT]GC
718 GCTGC
FokI 735 CATCC GGATG(9/13)
853 CATCC
ItaI 362 GCTGC GC^[ACGT]GC
718 GCTGC
MluNI 263 TGGCCA TGG^CCA
446 TGGCCA
MscI 263 TGGCCA TGG^CCA
446 TGGCCA
MslI 420 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
490 CATCTCTGTG
TfiI 76 GAATC G^A[AT]TC
921 GAATC
TseI 362 GCTGC G^C[AT]GC
718 GCTGC
Tsp509I 5 AATT ^AATT
107 AATT
XcmI 227 CCAGCCCCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
756 CCACAGTGCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BanII BmyI BsgI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
HaeIII HinfI MaeIII MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
Tsp45I TspRI