Restriction map for
pFN21AE5396
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 765 GTAC GT^AC
ApaI 502 GGGCCC GGGCC^C
AspHI 100 GTGCTC G[AT]GC[AT]^C
AvaII 687 GGACC G^G[AT]CC
BanI 4 GGTGCC G^G[CT][GA]CC
BanII 502 GGGCCC G[GA]GC[CT]^C
BglII 326 AGATCT A^GATCT
BsaAI 721 CACGTA [CT]AC^GT[GA]
BsaBI 532 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 580 GGTCTC GGTCTC(1/5)
BsiHKAI 100 GTGCTC G[AT]GC[AT]^C
BsmFI 773 GTCCC GGGAC(10/14)
BsmI 456 GCATTC GAATGC(1/-1)
Bsp1407I 764 TGTACA T^GTACA
BspLU11I 709 ACATGT A^CATGT
BsrFI 689 ACCGGC [GA]^CCGG[CT]
BsrGI 764 TGTACA T^GTACA
BstYI 326 AGATCT [GA]^GATC[CT]
Cfr10I 689 ACCGGC [GA]^CCGG[CT]
DrdI 153 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 344 CCATGG C^C[GA][CT]GG
EcoT14I 344 CCATGG C^C[AT][AT]GG
FauI 427 GCGGG CCCGC(4/6)
HphI 134 TCACC GGTGA(8/7)
MaeII 722 ACGT A^CGT
MaeIII 19 GTGAC ^GT[ACGT]AC
MamI 532 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 326 AGATCT [GA]^GATC[CT]
MseI 815 TTAA T^TAA
MwoI 828 GCCACCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 344 CCATGG C^CATGG
NdeI 204 CATATG CA^TATG
NheI 423 GCTAGC G^CTAGC
PstI 466 CTGCAG CTGCA^G
PvuII 487 CAGCTG CAG^CTG
RsaI 765 GTAC GT^AC
SmlI 547 CTCAAG C^T[CT][GA]AG
SspBI 764 TGTACA T^GTACA
StyI 344 CCATGG C^C[AT][AT]GG
Tru9I 815 TTAA T^TAA
Tsp45I 19 GTGAC ^GT[GC]AC
XcmI 306 CCACTTCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 326 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 709 ACATGT A^C[GA][CT]GT
720 ACACGT
BalI 365 TGGCCA TGG^CCA
593 TGGCCA
BfaI 146 CTAG C^TAG
424 CTAG
BmyI 100 GTGCTC G[AGT]GC[ACT]^C
502 GGGCCC
BseRI 475 CTCCTC GAGGAG(10/8)
909 GAGGAG
BsgI 382 CTGCAC GTGCAG(16/14)
560 GTGCAG
Bsp1286I 100 GTGCTC G[AGT]GC[ACT]^C
502 GGGCCC
BsrI 186 CCAGT ACTGG(1/-1)
675 CCAGT
BstXI 306 CCACTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCACCACCCTGG
DraII 376 AGGCCCC [GA]G^G[ACGT]CC[CT]
430 GGGGCCT
EaeI 365 TGGCCA [CT]^GGCC[GA]
593 TGGCCA
EcoO109I 376 AGGCCCC [GA]G^G[ACGT]CC[CT]
430 GGGGCCT
HapII 408 CCGG C^CGG
690 CCGG
HpaII 408 CCGG C^CGG
690 CCGG
MaeI 146 CTAG C^TAG
424 CTAG
MluNI 365 TGGCCA TGG^CCA
593 TGGCCA
MscI 365 TGGCCA TGG^CCA
593 TGGCCA
MspA1I 487 CAGCTG C[AC]G^C[GT]G
600 CAGCGG
MspI 408 CCGG C^CGG
690 CCGG
NspI 70 ACATGC [GA]CATG^[CT]
709 ACATGT
SfcI 372 CTGTAG C^T[GA][CT]AG
466 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmAI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII ItaI MboI
MboII MnlI MslI MvaI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI TspRI