Restriction map for
pFN21AE5379
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI HaeII HapII HgaI
Hin1I HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MroI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 733 GTCGAC GT^[AC][GT]AC
AfaI 96 GTAC GT^AC
AspHI 154 GTGCTC G[AT]GC[AT]^C
AvaII 516 GGACC G^G[AT]CC
AviII 559 TGCGCA TGC^GCA
BglII 203 AGATCT A^GATCT
BmyI 154 GTGCTC G[AGT]GC[ACT]^C
BpmI 547 CTGGAG CTGGAG(16/14)
BsaI 418 GGTCTC GGTCTC(1/5)
BsaJI 758 CCTTGG C^C[ACGT][ACGT]GG
BsgI 658 CTGCAC GTGCAG(16/14)
BsiHKAI 154 GTGCTC G[AT]GC[AT]^C
BsmAI 419 GTCTC GTCTC(1/5)
Bsp1286I 154 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 95 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BsrDI 116 GCAATG GCAATG(2/0)
BsrGI 95 TGTACA T^GTACA
BsrI 541 CCAGT ACTGG(1/-1)
BssKI 141 CCTGG ^CC[ACGT]GG
BssSI 424 CTCGTG CACGAG(-5/-1)
BstEII 132 GGTGACC G^GT[ACGT]ACC
BstNI 141 CCTGG CC^[AT]GG
BstPI 132 GGTGACC G^GT[ACGT]ACC
BstYI 203 AGATCT [GA]^GATC[CT]
CfoI 560 GCGC GCG^C
DraIII 538 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 132 GGTGACC G^GT[ACGT]ACC
EcoRII 141 CCTGG ^CC[AT]GG
EcoT14I 758 CCTTGG C^C[AT][AT]GG
FauI 443 GCGGG CCCGC(4/6)
FspI 559 TGCGCA TGC^GCA
HhaI 560 GCGC GCG^C
HinP1I 560 GCGC G^CGC
HincII 733 GTCGAC GT[CT]^[GA]AC
HindII 733 GTCGAC GT[CT]^[GA]AC
HindIII 232 AAGCTT A^AGCTT
HinfI 244 GACTC G^A[ACGT]TC
MflI 203 AGATCT [GA]^GATC[CT]
MseI 838 TTAA T^TAA
MspA1I 101 CAGCTG C[AC]G^C[GT]G
MvaI 141 CCTGG CC^[AT]GG
PflMI 675 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 244 GACTC GAGTC(4/5)
PstI 791 CTGCAG CTGCA^G
PvuII 101 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
RsaI 96 GTAC GT^AC
SalI 733 GTCGAC G^TCGAC
ScrFI 141 CCTGG CC^[ACGT]GG
SfcI 791 CTGCAG C^T[GA][CT]AG
SmlI 274 CTCAAG C^T[CT][GA]AG
SspBI 95 TGTACA T^GTACA
StuI 326 AGGCCT AGG^CCT
StyI 758 CCTTGG C^C[AT][AT]GG
TaqI 734 TCGA T^CGA
Tru9I 838 TTAA T^TAA
TthHB8I 734 TCGA T^CGA
Van91I 675 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 203 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 7 enzymes) #
###################################
BclI 587 TGATCA T^GATCA
650 TGATCA
BsmI 864 GAATGC GAATGC(1/-1)
456 GCATTC
BstF5I 138 CATCC GGATG(2/0)
654 CATCC
BstXI 306 CCATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
506 CCAACATCATGG
FbaI 587 TGATCA T^GATCA
650 TGATCA
FokI 138 CATCC GGATG(9/13)
654 CATCC
XcmI 306 CCATTTCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
405 CCAGCGAATGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BalI BbvI BseRI
BsiYI BslI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I Fnu4HI
HaeIII HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MslI
NdeII NlaIII NlaIV Sau3AI Sau96I
TseI Tsp45I Tsp509I TspRI