##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MamI        MluI        MroI        
MspI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI           58  GCGG                 CCGC(-3/-1)
AcsI          232  AAATTC               [GA]^AATT[CT]
ApoI          232  AAATTC               [GA]^AATT[CT]
BamHI          51  GGATCC               G^GATCC
BbsI          616  GTCTTC               GAAGAC(2/6)
BbvI          185  GCTGC                GCAGC(8/12)
BpmI          535  CTCCAG               CTGGAG(16/14)
BpuAI         616  GTCTTC               GAAGAC(2/6)
BsaAI         361  TACGTG               [CT]AC^GT[GA]
BsmFI         503  GTCCC                GGGAC(10/14)
BsmI          864  GAATGC               GAATGC(1/-1)
BspMI         750  ACCTGC               ACCTGC(4/8)
BsrDI         116  GCAATG               GCAATG(2/0)
BstF5I        483  CATCC                GGATG(2/0)
DsaI          104  CCACGG               C^C[GA][CT]GG
EarI          380  CTCTTC               CTCTTC(1/4)
EcoNI          42  CCTTTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       194  CCTTGG               C^C[AT][AT]GG
Fnu4HI        185  GCTGC                GC^[ACGT]GC
FokI          483  CATCC                GGATG(9/13)
HindIII       550  AAGCTT               A^AGCTT
ItaI          185  GCTGC                GC^[ACGT]GC
Ksp632I       380  CTCTTC               CTCTTC(1/4)
MaeII         362  ACGT                 A^CGT
MfeI          487  CAATTG               C^AATTG
MseI          260  TTAA                 T^TAA
MspA1I         56  CAGCGG               C[AC]G^C[GT]G
MunI          487  CAATTG               C^AATTG
NspI          767  ACATGC               [GA]CATG^[CT]
PstI          680  CTGCAG               CTGCA^G
SfcI          680  CTGCAG               C^T[GA][CT]AG
SmlI          666  CTTGAG               C^T[CT][GA]AG
StyI          194  CCTTGG               C^C[AT][AT]GG
Tru9I         260  TTAA                 T^TAA
TseI          185  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          184  AGCT                 AG^CT
              551  AGCT                
AvaII         502  GGTCC                G^G[AT]CC
              748  GGACC               
BsgI          558  CTGCAC               GTGCAG(16/14)
               61  GTGCAG              
BsrI           37  ACTGG                ACTGG(1/-1)
                5  CCAGT               
BstYI          51  GGATCC               [GA]^GATC[CT]
              326  AGATCC              
Cfr13I        502  GGTCC                G^G[ACGT]CC
              748  GGACC               
HincII        407  GTCAAC               GT[CT]^[GA]AC
              661  GTCAAC              
HindII        407  GTCAAC               GT[CT]^[GA]AC
              661  GTCAAC              
MflI           51  GGATCC               [GA]^GATC[CT]
              326  AGATCC              
PleI          288  GACTC                GAGTC(4/5)
              153  GAGTC               
Sau96I        502  GGTCC                G^G[ACGT]CC
              748  GGACC               
XhoII          51  GGATCC               [GA]^GATC[CT]
              326  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AfaI        AlwI        BalI        BsaJI       BsiYI       
BslI        BssKI       BstNI       BstXI       Cac8I       
DdeI        DpnII       EaeI        EcoRII      HaeIII      
HinfI       HphI        MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MvaI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
ScrFI       TfiI        Tsp45I      Tsp509I     TspRI