Restriction map for
pFN21AE3174
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AspEI AspI AvaII AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KspI MaeII MamI
MluI MroI MspA1I MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 599 GGTACC G^GTACC
AseI 456 ATTAAT AT^TAAT
AsnI 456 ATTAAT AT^TAAT
Asp700I 235 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 599 GGTACC G^GTACC
AvaI 134 CCCGGG C^[CT]CG[GA]G
BalI 85 TGGCCA TGG^CCA
BanI 599 GGTACC G^G[CT][GA]CC
BbsI 909 GAAGAC GAAGAC(2/6)
BglII 175 AGATCT A^GATCT
BlpI 1190 GCTCAGC GC^T[ACGT]AGC
BpmI 467 CTGGAG CTGGAG(16/14)
Bpu1102I 1190 GCTCAGC GC^T[ACGT]AGC
BpuAI 909 GAAGAC GAAGAC(2/6)
BseRI 682 GAGGAG GAGGAG(10/8)
BsgI 472 GTGCAG GTGCAG(16/14)
BsmFI 903 GGGAC GGGAC(10/14)
BsoBI 134 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1167 TGTACA T^GTACA
BspMI 596 GCAGGT ACCTGC(4/8)
BsrGI 1167 TGTACA T^GTACA
CelII 1190 GCTCAGC GC^T[ACGT]AGC
CfoI 1188 GCGC GCG^C
DrdI 932 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 85 TGGCCA [CT]^GGCC[GA]
EcoT14I 350 CCTTGG C^C[AT][AT]GG
EcoT22I 374 ATGCAT ATGCA^T
HaeII 1187 GGCGCT [GA]GCGC^[CT]
HapII 135 CCGG C^CGG
HhaI 1188 GCGC GCG^C
HinP1I 1188 GCGC G^CGC
HindIII 1198 AAGCTT A^AGCTT
HpaII 135 CCGG C^CGG
KpnI 599 GGTACC GGTAC^C
MluNI 85 TGGCCA TGG^CCA
MscI 85 TGGCCA TGG^CCA
MspI 135 CCGG C^CGG
MwoI 431 GCCAGTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 599 GGTACC GG[ACGT]^[ACGT]CC
NsiI 374 ATGCAT ATGCA^T
PleI 328 GAGTC GAGTC(4/5)
PshBI 456 ATTAAT AT^TAAT
PstI 319 CTGCAG CTGCA^G
SexAI 933 ACCTGGT A^CC[AT]GGT
SmaI 134 CCCGGG CCC^GGG
SphI 690 GCATGC GCATG^C
SspBI 1167 TGTACA T^GTACA
StyI 350 CCTTGG C^C[AT][AT]GG
TfiI 58 GAATC G^A[AT]TC
Tsp45I 252 GTCAC ^GT[GC]AC
TspRI 615 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 276 TCTAGA T^CTAGA
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
XmnI 235 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 504 GGATC GGATC(4/5)
975 GGATC
AspHI 883 GAGCTC G[AT]GC[AT]^C
1109 GAGCTC
BanII 883 GAGCTC G[GA]GC[CT]^C
1109 GAGCTC
BciVI 9 GTATCC GTATCC(6/5)
402 GTATCC
BcnI 134 CCCGG CC^[GC]GG
135 CCGGG
BfaI 277 CTAG C^TAG
970 CTAG
BsaI 359 GAGACC GGTCTC(1/5)
930 GAGACC
BsiHKAI 883 GAGCTC G[AT]GC[AT]^C
1109 GAGCTC
BsmI 37 GAATGC GAATGC(1/-1)
633 GAATGC
BsrI 220 CCAGT ACTGG(1/-1)
432 CCAGT
BstYI 175 AGATCT [GA]^GATC[CT]
504 GGATCT
Cfr13I 429 GGGCC G^G[ACGT]CC
568 GGCCC
EarI 860 CTCTTC CTCTTC(1/4)
1106 GAAGAG
EcoNI 70 CCTAATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
538 CCTGAAAGAGG
HinfI 58 GAATC G^A[ACGT]TC
328 GAGTC
HphI 253 TCACC GGTGA(8/7)
743 TCACC
Ksp632I 860 CTCTTC CTCTTC(1/4)
1106 GAAGAG
MaeI 277 CTAG C^TAG
970 CTAG
MaeIII 168 GTTAC ^GT[ACGT]AC
252 GTCAC
MfeI 713 CAATTG C^AATTG
1063 CAATTG
MflI 175 AGATCT [GA]^GATC[CT]
504 GGATCT
MslI 579 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
867 CATATTTGTG
MunI 713 CAATTG C^AATTG
1063 CAATTG
NciI 134 CCCGG CC^[GC]GG
135 CCGGG
NspI 608 ACATGC [GA]CATG^[CT]
690 GCATGC
SacI 883 GAGCTC GAGCT^C
1109 GAGCTC
SapI 1106 GAAGAGC GCTCTTC(1/4)
859 GCTCTTC
Sau96I 429 GGGCC G^G[ACGT]CC
568 GGCCC
SmlI 155 CTCAAG C^T[CT][GA]AG
179 CTCAAG
XhoII 175 AGATCT [GA]^GATC[CT]
504 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII ItaI
MboI MboII MnlI MseI MvaI
NdeII NlaIII RsaI Sau3AI ScrFI
SfaNI SfcI Tru9I TseI Tsp509I