Restriction map for
pFN21AE4259
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcnI BfrI BglI
BlnI BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI BstEII BstPI Bsu36I
ClaI CpoI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
MaeII MluI MluNI MroI MscI
NarI NciI NheI NotI NruI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 636 GTATAC GT^[AC][GT]AC
AccII 63 CGCG CG^CG
AhdI 2940 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 546 GGATC GGATC(4/5)
AseI 985 ATTAAT AT^TAAT
AsnI 985 ATTAAT AT^TAAT
AspEI 2940 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 956 CTCGAG C^[CT]CG[GA]G
BanI 34 GGCACC G^G[CT][GA]CC
BbsI 1108 GAAGAC GAAGAC(2/6)
BcgI 417 CGATAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3046 GGATAC GTATCC(6/5)
BlpI 2758 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2758 GCTAAGC GC^T[ACGT]AGC
BpuAI 1108 GAAGAC GAAGAC(2/6)
BsaBI 2869 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 956 CTCGAG C^[CT]CG[GA]G
BspHI 2493 TCATGA T^CATGA
BspMI 163 GCAGGT ACCTGC(4/8)
BsrBI 496 GAGCGG CCGCTC(-3/-3)
BsrFI 2586 GCCGGC [GA]^CCGG[CT]
Bst1107I 636 GTATAC GTA^TAC
BstBI 2333 TTCGAA TT^CGAA
BstUI 63 CGCG CG^CG
BstZ17I 636 GTATAC GTA^TAC
CelII 2758 GCTAAGC GC^T[ACGT]AGC
Cfr10I 2586 GCCGGC [GA]^CCGG[CT]
DraII 23 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2940 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 51 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 23 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 336 ATGCAT ATGCA^T
FauI 30 GCGGG CCCGC(4/6)
HaeII 68 AGCGCC [GA]GCGC^[CT]
HapII 2587 CCGG C^CGG
HpaII 2587 CCGG C^CGG
KspI 62 CCGCGG CCGC^GG
MamI 2869 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 2587 CCGG C^CGG
MvnI 63 CGCG CG^CG
NaeI 2586 GCCGGC GCC^GGC
NgoMIV 2586 GCCGGC G^CCGGC
NsiI 336 ATGCAT ATGCA^T
NspV 2333 TTCGAA TT^CGAA
PaeR7I 956 CTCGAG C^TCGAG
PshBI 985 ATTAAT AT^TAAT
PstI 2239 CTGCAG CTGCA^G
PvuII 139 CAGCTG CAG^CTG
RcaI 2493 TCATGA T^CATGA
SacI 310 GAGCTC GAGCT^C
SacII 62 CCGCGG CCGC^GG
SfuI 2333 TTCGAA TT^CGAA
XhoI 956 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1364 ACATGT A^C[GA][CT]GT
1987 ACATGT
AvaII 3 GGACC G^G[AT]CC
42 GGACC
BanII 310 GAGCTC G[GA]GC[CT]^C
1292 GAGCCC
BfaI 436 CTAG C^TAG
1405 CTAG
BpmI 118 CTCCAG CTGGAG(16/14)
3062 CTCCAG
BsiYI 51 CCTGCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3097 CCAGCACCAGG
BslI 51 CCTGCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3097 CCAGCACCAGG
BsmI 338 GCATTC GAATGC(1/-1)
2270 GCATTC
BspLU11I 1364 ACATGT A^CATGT
1987 ACATGT
BstAPI 525 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1813 GCAGTTACTGC
BstXI 367 CCAAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2005 CCACAGATGTGG
CfoI 69 GCGC GCG^C
1975 GCGC
HhaI 69 GCGC GCG^C
1975 GCGC
HinP1I 69 GCGC G^CGC
1975 GCGC
HincII 1991 GTCAAC GT[CT]^[GA]AC
2473 GTTGAC
HindII 1991 GTCAAC GT[CT]^[GA]AC
2473 GTTGAC
HphI 2770 GGTGA GGTGA(8/7)
1010 TCACC
MaeI 436 CTAG C^TAG
1405 CTAG
MfeI 1841 CAATTG C^AATTG
2357 CAATTG
MunI 1841 CAATTG C^AATTG
2357 CAATTG
NcoI 38 CCATGG C^CATGG
766 CCATGG
NdeI 1300 CATATG CA^TATG
2624 CATATG
SapI 3005 GAAGAGC GCTCTTC(1/4)
1633 GCTCTTC
SfcI 1200 CTGTAG C^T[GA][CT]AG
2239 CTGCAG
SmlI 956 CTCGAG C^T[CT][GA]AG
1894 CTTGAG
SspI 280 AATATT AAT^ATT
2741 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AspHI BbvI BclI
BglII BmyI BsaJI BseRI BsiHKAI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI