Restriction map for
pFN21AE6259
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI HphI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 590 GTCTAC GT^[AC][GT]AC
AlwNI 548 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 505 GAGCAC G[AT]GC[AT]^C
AvaII 235 GGTCC G^G[AT]CC
BanI 197 GGCGCC G^G[CT][GA]CC
BbeI 197 GGCGCC GGCGC^C
BcnI 476 CCCGG CC^[GC]GG
BfaI 511 CTAG C^TAG
BsaI 40 GAGACC GGTCTC(1/5)
BsgI 97 GTGCAG GTGCAG(16/14)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsiHKAI 505 GAGCAC G[AT]GC[AT]^C
BspMI 125 ACCTGC ACCTGC(4/8)
BsrFI 638 ACCGGC [GA]^CCGG[CT]
BstAPI 90 GCAGGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 336 CCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 90 GCAGGC GC[ACGT]^[ACGT]GC
Cfr10I 638 ACCGGC [GA]^CCGG[CT]
DraIII 508 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 30 CGGCCG [CT]^GGCC[GA]
EagI 30 CGGCCG C^GGCCG
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
EcoNI 18 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 51 GCGGG CCCGC(4/6)
HgaI 61 GCGTC GACGC(5/10)
HincII 367 GTTGAC GT[CT]^[GA]AC
HindII 367 GTTGAC GT[CT]^[GA]AC
HindIII 463 AAGCTT A^AGCTT
KasI 197 GGCGCC G^GCGCC
MaeI 511 CTAG C^TAG
MaeIII 63 GTCAC ^GT[ACGT]AC
MslI 445 CACAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 551 CAGCTG C[AC]G^C[GT]G
NarI 197 GGCGCC GG^CGCC
NciI 476 CCCGG CC^[GC]GG
NotI 29 GCGGCCGC GC^GGCCGC
PstI 190 CTGCAG CTGCA^G
PvuII 551 CAGCTG CAG^CTG
SfoI 197 GGCGCC GGC^GCC
SspI 184 AATATT AAT^ATT
Tsp45I 63 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 60 GGCGTC G[GA]^CG[CT]C
197 GGCGCC
BbsI 654 GAAGAC GAAGAC(2/6)
703 GAAGAC
BclI 515 TGATCA T^GATCA
644 TGATCA
BpuAI 654 GAAGAC GAAGAC(2/6)
703 GAAGAC
BsaHI 60 GGCGTC G[GA]^CG[CT]C
197 GGCGCC
BseRI 16 CTCCTC GAGGAG(10/8)
307 GAGGAG
BsrI 522 ACTGG ACTGG(1/-1)
336 CCAGT
BstNI 135 CCTGG CC^[AT]GG
537 CCTGG
CfoI 78 GCGC GCG^C
198 GCGC
EcoRII 135 CCTGG ^CC[AT]GG
537 CCTGG
FbaI 515 TGATCA T^GATCA
644 TGATCA
HaeII 77 AGCGCC [GA]GCGC^[CT]
197 GGCGCC
HhaI 78 GCGC GCG^C
198 GCGC
Hin1I 60 GGCGTC G[GA]^CG[CT]C
197 GGCGCC
HinP1I 78 GCGC G^CGC
198 GCGC
MvaI 135 CCTGG CC^[AT]GG
537 CCTGG
SfaNI 480 GCATC GCATC(5/9)
617 GCATC
SfcI 190 CTGCAG C^T[GA][CT]AG
592 CTACAG
SmlI 145 CTCAAG C^T[CT][GA]AG
424 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I Cfr13I DdeI
DpnII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MspI MwoI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI StyI TseI TspRI