Restriction map for
pFN21AE5478
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI StyI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 886 GCGG CCGC(-3/-1)
AgeI 410 ACCGGT A^CCGGT
AlwI 120 GATCC GGATC(4/5)
AlwNI 118 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 488 GGTCC G^G[AT]CC
BalI 638 TGGCCA TGG^CCA
BanII 944 GGGCTC G[GA]GC[CT]^C
BbvI 387 GCTGC GCAGC(8/12)
BglI 496 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 255 AGATCT A^GATCT
BsaWI 410 ACCGGT [AT]^CCGG[AT]
Bsp1407I 373 TGTACA T^GTACA
BspHI 452 TCATGA T^CATGA
BsrFI 410 ACCGGT [GA]^CCGG[CT]
BsrGI 373 TGTACA T^GTACA
CfoI 385 GCGC GCG^C
Cfr10I 410 ACCGGT [GA]^CCGG[CT]
DraII 487 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 796 CTCTTC CTCTTC(1/4)
EcoO109I 487 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 867 GATATC GAT^ATC
Fnu4HI 387 GCTGC GC^[ACGT]GC
HapII 411 CCGG C^CGG
HhaI 385 GCGC GCG^C
HinP1I 385 GCGC G^CGC
HpaII 411 CCGG C^CGG
ItaI 387 GCTGC GC^[ACGT]GC
Ksp632I 796 CTCTTC CTCTTC(1/4)
MaeIII 889 GTGAC ^GT[ACGT]AC
MluNI 638 TGGCCA TGG^CCA
MscI 638 TGGCCA TGG^CCA
MspA1I 884 CAGCGG C[AC]G^C[GT]G
MspI 411 CCGG C^CGG
NspI 824 ACATGC [GA]CATG^[CT]
PinAI 410 ACCGGT A^CCGGT
PpuMI 487 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 452 TCATGA T^CATGA
SfaNI 729 GATGC GCATC(5/9)
SspBI 373 TGTACA T^GTACA
TseI 387 GCTGC G^C[AT]GC
Tsp45I 889 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 374 GTAC GT^AC
438 GTAC
BmyI 503 GGGCAC G[AGT]GC[ACT]^C
944 GGGCTC
BsaJI 90 CCTGGG C^C[ACGT][ACGT]GG
491 CCTCGG
BsmFI 555 GGGAC GGGAC(10/14)
280 GTCCC
Bsp1286I 503 GGGCAC G[AGT]GC[ACT]^C
944 GGGCTC
BstYI 119 AGATCC [GA]^GATC[CT]
255 AGATCT
Cfr13I 488 GGTCC G^G[ACGT]CC
672 GGCCC
DdeI 235 CTTAG C^T[ACGT]AG
949 CTCAG
DpnII 120 GATC ^GATC
256 GATC
EaeI 494 CGGCCA [CT]^GGCC[GA]
638 TGGCCA
HphI 888 GGTGA GGTGA(8/7)
818 TCACC
MboI 120 GATC ^GATC
256 GATC
MflI 119 AGATCC [GA]^GATC[CT]
255 AGATCT
NdeII 120 GATC ^GATC
256 GATC
NlaIV 672 GGCCCC GG[ACGT]^[ACGT]CC
772 GGCTCC
PleI 749 GACTC GAGTC(4/5)
891 GACTC
RsaI 374 GTAC GT^AC
438 GTAC
Sau3AI 120 GATC ^GATC
256 GATC
Sau96I 488 GGTCC G^G[ACGT]CC
672 GGCCC
SpeI 507 ACTAGT A^CTAGT
957 ACTAGT
SspI 706 AATATT AAT^ATT
813 AATATT
TfiI 202 GATTC G^A[AT]TC
687 GATTC
Tsp509I 351 AATT ^AATT
912 AATT
XcmI 641 CCATCTTTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
726 CCAGATGCAGTCTGG
XhoII 119 AGATCC [GA]^GATC[CT]
255 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BfaI BsiYI BslI BsmAI
BsrI BssKI BstNI Cac8I EcoRII
HaeIII HinfI MaeI MboII MnlI
MslI MvaI MwoI NlaIII ScrFI
SmlI TspRI