Restriction map for
pFN21AE5376
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BcgI BclI BcnI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 324 CCGC CCGC(-3/-1)
AfaI 138 GTAC GT^AC
AflIII 679 ACATGT A^C[GA][CT]GT
AlwNI 894 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 763 CCTGAGG CC^T[ACGT]AGG
Asp700I 483 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 209 GGACC G^G[AT]CC
BalI 332 TGGCCA TGG^CCA
BanI 469 GGCACC G^G[CT][GA]CC
BciVI 902 GTATCC GTATCC(6/5)
BglI 385 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 548 AGATCT A^GATCT
BmyI 193 GTGCCC G[AGT]GC[ACT]^C
BpmI 166 CTGGAG CTGGAG(16/14)
BsaI 873 GGTCTC GGTCTC(1/5)
Bsp1286I 193 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 679 ACATGT A^CATGT
BsrDI 621 CATTGC GCAATG(2/0)
BssSI 380 CTCGTG CACGAG(-5/-1)
BstF5I 633 CATCC GGATG(2/0)
Bsu36I 763 CCTGAGG CC^T[ACGT]AGG
DsaI 311 CCATGG C^C[GA][CT]GG
EaeI 332 TGGCCA [CT]^GGCC[GA]
Eco81I 763 CCTGAGG CC^T[ACGT]AGG
FokI 633 CATCC GGATG(9/13)
HapII 741 CCGG C^CGG
HpaII 741 CCGG C^CGG
MluNI 332 TGGCCA TGG^CCA
MscI 332 TGGCCA TGG^CCA
MseI 724 TTAA T^TAA
MspI 741 CCGG C^CGG
NcoI 311 CCATGG C^CATGG
NdeI 317 CATATG CA^TATG
NspI 679 ACATGT [GA]CATG^[CT]
PflMI 460 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 208 GGGACCC [GA]G^G[AT]CC[CT]
PstI 339 CTGCAG CTGCA^G
RsaI 138 GTAC GT^AC
SapI 447 GCTCTTC GCTCTTC(1/4)
SexAI 65 ACCTGGT A^CC[AT]GGT
SmlI 637 CTCAAG C^T[CT][GA]AG
Tru9I 724 TTAA T^TAA
Tsp509I 757 AATT ^AATT
TspRI 795 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 460 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 483 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 585 GATCC GGATC(4/5)
642 GATCC
BbvCI 861 CCTCAGC CCTCAGC(-5/-2)
513 GCTGAGG
BsgI 433 GTGCAG GTGCAG(16/14)
527 GTGCAG
BsmAI 703 GTCTC GTCTC(1/5)
874 GTCTC
BsmFI 208 GGGAC GGGAC(10/14)
344 GTCCC
BsmI 292 GAATGC GAATGC(1/-1)
855 GAATGC
BspHI 365 TCATGA T^CATGA
581 TCATGA
BsrI 390 ACTGG ACTGG(1/-1)
358 CCAGT
BstEII 69 GGTCACC G^GT[ACGT]ACC
102 GGTTACC
BstPI 69 GGTCACC G^GT[ACGT]ACC
102 GGTTACC
BstYI 548 AGATCT [GA]^GATC[CT]
641 AGATCC
Cac8I 216 GCTTGC GC[ACGT]^[ACGT]GC
593 GCTTGC
Cfr13I 209 GGACC G^G[ACGT]CC
882 GGCCC
DraII 208 GGGACCC [GA]G^G[ACGT]CC[CT]
881 AGGCCCT
EarI 9 CTCTTC CTCTTC(1/4)
448 CTCTTC
EcoO109I 208 GGGACCC [GA]G^G[ACGT]CC[CT]
881 AGGCCCT
EcoO65I 69 GGTCACC G^GT[ACGT]ACC
102 GGTTACC
EcoT14I 243 CCTTGG C^C[AT][AT]GG
311 CCATGG
HinfI 378 GACTC G^A[ACGT]TC
804 GACTC
Ksp632I 9 CTCTTC CTCTTC(1/4)
448 CTCTTC
MflI 548 AGATCT [GA]^GATC[CT]
641 AGATCC
PleI 378 GACTC GAGTC(4/5)
804 GACTC
RcaI 365 TCATGA T^CATGA
581 TCATGA
Sau96I 209 GGACC G^G[ACGT]CC
882 GGCCC
StyI 243 CCTTGG C^C[AT][AT]GG
311 CCATGG
Tsp45I 70 GTCAC ^GT[GC]AC
497 GTGAC
XhoII 548 AGATCT [GA]^GATC[CT]
641 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BsiYI
BslI BssKI BstNI BstXI DdeI
DpnII EcoRII Fnu4HI HaeIII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfcI
TseI