Restriction map for
pFN21AE6047
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BsaAI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII HapII HindIII HpaI
HpaII KasI KspI MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 413 GGTACC G^GTACC
AcyI 15 GGCGTC G[GA]^CG[CT]C
AflIII 739 ACATGT A^C[GA][CT]GT
AlwI 840 GATCC GGATC(4/5)
Asp700I 333 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 413 GGTACC G^GTACC
BanI 413 GGTACC G^G[CT][GA]CC
BanII 642 GGGCTC G[GA]GC[CT]^C
BbsI 457 GTCTTC GAAGAC(2/6)
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
BbvI 90 GCAGC GCAGC(8/12)
BclI 158 TGATCA T^GATCA
BfaI 452 CTAG C^TAG
BlpI 207 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 207 GCTCAGC GC^T[ACGT]AGC
BpuAI 457 GTCTTC GAAGAC(2/6)
BsaHI 15 GGCGTC G[GA]^CG[CT]C
BsmAI 46 GTCTC GTCTC(1/5)
BsmI 474 GAATGC GAATGC(1/-1)
BspHI 329 TCATGA T^CATGA
BspLU11I 739 ACATGT A^CATGT
BspMI 203 ACCTGC ACCTGC(4/8)
Cac8I 307 GCCTGC GC[ACGT]^[ACGT]GC
CelII 207 GCTCAGC GC^T[ACGT]AGC
CfoI 478 GCGC GCG^C
EarI 121 CTCTTC CTCTTC(1/4)
FbaI 158 TGATCA T^GATCA
HgaI 16 GCGTC GACGC(5/10)
HhaI 478 GCGC GCG^C
Hin1I 15 GGCGTC G[GA]^CG[CT]C
HinP1I 478 GCGC G^CGC
HincII 798 GTTGAC GT[CT]^[GA]AC
HindII 798 GTTGAC GT[CT]^[GA]AC
KpnI 413 GGTACC GGTAC^C
Ksp632I 121 CTCTTC CTCTTC(1/4)
MaeI 452 CTAG C^TAG
MseI 602 TTAA T^TAA
MspA1I 169 CAGCTG C[AC]G^C[GT]G
NspI 739 ACATGT [GA]CATG^[CT]
PleI 352 GAGTC GAGTC(4/5)
PstI 940 CTGCAG CTGCA^G
PvuII 169 CAGCTG CAG^CTG
RcaI 329 TCATGA T^CATGA
SfaNI 551 GCATC GCATC(5/9)
SfcI 940 CTGCAG C^T[GA][CT]AG
StuI 176 AGGCCT AGG^CCT
TaqI 292 TCGA T^CGA
Tru9I 602 TTAA T^TAA
TseI 90 GCAGC G^C[AT]GC
Tsp45I 18 GTCAC ^GT[GC]AC
TthHB8I 292 TCGA T^CGA
XcmI 615 CCAATTTGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 333 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 52 GAATTC [GA]^AATT[CT]
926 GAATTC
AfaI 198 GTAC GT^AC
414 GTAC
AlwNI 44 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
190 CAGCCCCTG
ApoI 52 GAATTC [GA]^AATT[CT]
926 GAATTC
AspHI 235 GTGCTC G[AT]GC[AT]^C
735 GAGCAC
BpmI 316 CTCCAG CTGGAG(16/14)
172 CTGGAG
BsaBI 159 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
321 GATGTTCATC
BseRI 58 CTCCTC GAGGAG(10/8)
214 CTCCTC
BsiHKAI 235 GTGCTC G[AT]GC[AT]^C
735 GAGCAC
BsmFI 40 GTCCC GGGAC(10/14)
823 GTCCC
BsrI 95 ACTGG ACTGG(1/-1)
43 CCAGT
EcoRI 52 GAATTC G^AATTC
926 GAATTC
Fnu4HI 90 GCAGC GC^[ACGT]GC
718 GCGGC
ItaI 90 GCAGC GC^[ACGT]GC
718 GCGGC
MaeIII 18 GTCAC ^GT[ACGT]AC
936 GTTAC
MamI 159 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
321 GATGTTCATC
PflMI 269 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
615 CCAATTTGTGG
PpuMI 260 AGGTCCT [GA]G^G[AT]CC[CT]
821 AGGTCCC
RsaI 198 GTAC GT^AC
414 GTAC
Van91I 269 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
615 CCAATTTGTGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BalI BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstXI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FokI HaeIII
HinfI HphI MaeII MboI MboII
MluNI MnlI MscI MslI MvaI
MwoI NcoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI StyI TfiI
Tsp509I TspRI