Restriction map for
pFN21AE5584
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BamHI BanI
BbeI BbsI BcgI BciVI BfrI
BglI BglII BlnI BpuAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MflI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp45I Tth111I XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 883 ACGTGT A^C[GA][CT]GT
AhdI 427 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 845 GGATC GGATC(4/5)
AocI 429 CCTCAGG CC^T[ACGT]AGG
AspEI 427 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 405 GGTCC G^G[AT]CC
BanII 786 GGGCTC G[GA]GC[CT]^C
BbrPI 882 CACGTG CAC^GTG
BbvCI 255 CCTCAGC CCTCAGC(-5/-2)
BbvI 234 GCAGC GCAGC(8/12)
BcnI 614 CCCGG CC^[GC]GG
BlpI 351 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 351 GCTCAGC GC^T[ACGT]AGC
BsaAI 882 CACGTG [CT]AC^GT[GA]
BsaI 129 GGTCTC GGTCTC(1/5)
BsmI 618 GAATGC GAATGC(1/-1)
BspHI 473 TCATGA T^CATGA
BsrDI 170 GCAATG GCAATG(2/0)
Bsu36I 429 CCTCAGG CC^T[ACGT]AGG
CelII 351 GCTCAGC GC^T[ACGT]AGC
DraI 15 TTTAAA TTT^AAA
DrdI 489 GACCATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 427 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 429 CCTCAGG CC^T[ACGT]AGG
Fnu4HI 234 GCAGC GC^[ACGT]GC
HapII 615 CCGG C^CGG
HpaII 615 CCGG C^CGG
ItaI 234 GCAGC GC^[ACGT]GC
MaeIII 704 GTAAC ^GT[ACGT]AC
MspA1I 313 CAGCTG C[AC]G^C[GT]G
MspI 615 CCGG C^CGG
NciI 614 CCCGG CC^[GC]GG
NlaIV 889 GGTTCC GG[ACGT]^[ACGT]CC
PmaCI 882 CACGTG CAC^GTG
PmlI 882 CACGTG CAC^GTG
PpuMI 404 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 699 CTGCAG CTGCA^G
PvuII 313 CAGCTG CAG^CTG
RcaI 473 TCATGA T^CATGA
SfcI 699 CTGCAG C^T[GA][CT]AG
SmlI 876 CTTGAG C^T[CT][GA]AG
TaqI 436 TCGA T^CGA
TfiI 1070 GAATC G^A[AT]TC
TseI 234 GCAGC G^C[AT]GC
TthHB8I 436 TCGA T^CGA
XbaI 73 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 196 GAATTC [GA]^AATT[CT]
965 GAATTC
ApoI 196 GAATTC [GA]^AATT[CT]
965 GAATTC
AspHI 379 GTGCTC G[AT]GC[AT]^C
879 GAGCAC
BclI 140 TGATCA T^GATCA
302 TGATCA
BfaI 74 CTAG C^TAG
590 CTAG
BsaBI 303 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
465 GATGTTCATC
BseRI 202 CTCCTC GAGGAG(10/8)
358 CTCCTC
BsiHKAI 379 GTGCTC G[AT]GC[AT]^C
879 GAGCAC
BsmFI 30 GGGAC GGGAC(10/14)
979 GTCCC
BspMI 347 ACCTGC ACCTGC(4/8)
1079 ACCTGC
BstXI 308 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
401 CCAAGGTCCTGG
DraII 41 AGGGCCT [GA]G^G[ACGT]CC[CT]
404 AGGTCCT
EcoO109I 41 AGGGCCT [GA]G^G[ACGT]CC[CT]
404 AGGTCCT
EcoRI 196 GAATTC G^AATTC
965 GAATTC
FbaI 140 TGATCA T^GATCA
302 TGATCA
HphI 305 TCACC GGTGA(8/7)
389 TCACC
MaeI 74 CTAG C^TAG
590 CTAG
MamI 303 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
465 GATGTTCATC
MseI 16 TTAA T^TAA
116 TTAA
MslI 504 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
510 CATGGTCATG
NcoI 278 CCATGG C^CATGG
491 CCATGG
SfaNI 160 GCATC GCATC(5/9)
695 GCATC
StuI 320 AGGCCT AGG^CCT
872 AGGCCT
Tru9I 16 TTAA T^TAA
116 TTAA
Tsp509I 197 AATT ^AATT
966 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwNI BalI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FokI HaeIII HinfI Ksp632I MaeII
MboI MboII MluNI MnlI MscI
MvaI MwoI NdeII NlaIII PflMI
PleI RsaI Sau3AI Sau96I ScrFI
StyI TspRI Van91I