Restriction map for
pFN21AE5950
##################################
# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI ItaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StyI SwaI TseI Tsp45I
TspRI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 167 GTCTAC GT^[AC][GT]AC
AciI 369 CCGC CCGC(-3/-1)
AcsI 459 AAATTT [GA]^AATT[CT]
AflIII 320 ACATGT A^C[GA][CT]GT
Alw44I 572 GTGCAC G^TGCAC
ApaLI 572 GTGCAC G^TGCAC
ApoI 459 AAATTT [GA]^AATT[CT]
AvaII 887 GGACC G^G[AT]CC
BanII 601 GGGCTC G[GA]GC[CT]^C
BciVI 431 GTATCC GTATCC(6/5)
BclI 149 TGATCA T^GATCA
BcnI 560 CCGGG CC^[GC]GG
BglI 559 GCCGGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 394 CTCCAG CTGGAG(16/14)
BsmAI 337 GAGAC GTCTC(1/5)
BsmFI 850 GTCCC GGGAC(10/14)
Bsp1407I 924 TGTACA T^GTACA
BspLU11I 320 ACATGT A^CATGT
BspMI 855 ACCTGC ACCTGC(4/8)
BsrGI 924 TGTACA T^GTACA
BstYI 896 AGATCC [GA]^GATC[CT]
Cfr13I 887 GGACC G^G[ACGT]CC
EarI 648 CTCTTC CTCTTC(1/4)
FbaI 149 TGATCA T^GATCA
HapII 560 CCGG C^CGG
HpaII 560 CCGG C^CGG
Ksp632I 648 CTCTTC CTCTTC(1/4)
MaeIII 472 GTAAC ^GT[ACGT]AC
MflI 896 AGATCC [GA]^GATC[CT]
MslI 733 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 560 CCGG C^CGG
MwoI 559 GCCGGGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 560 CCGGG CC^[GC]GG
NspI 320 ACATGT [GA]CATG^[CT]
PflMI 505 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 881 GAGTC GAGTC(4/5)
Sau96I 887 GGACC G^G[ACGT]CC
SfiI 558 GGCCGGGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 692 CTCAAG C^T[CT][GA]AG
SspBI 924 TGTACA T^GTACA
SspI 918 AATATT AAT^ATT
StuI 710 AGGCCT AGG^CCT
TaqI 669 TCGA T^CGA
TfiI 254 GAATC G^A[AT]TC
TthHB8I 669 TCGA T^CGA
Van91I 505 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 896 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 408 GATCC GGATC(4/5)
897 GATCC
AspHI 544 GAGCAC G[AT]GC[AT]^C
572 GTGCAC
BalI 194 TGGCCA TGG^CCA
795 TGGCCA
BsiHKAI 544 GAGCAC G[AT]GC[AT]^C
572 GTGCAC
BsrI 63 ACTGG ACTGG(1/-1)
396 CCAGT
BstNI 58 CCAGG CC^[AT]GG
192 CCTGG
EcoRII 58 CCAGG ^CC[AT]GG
192 CCTGG
HinfI 254 GAATC G^A[ACGT]TC
881 GAGTC
MluNI 194 TGGCCA TGG^CCA
795 TGGCCA
MscI 194 TGGCCA TGG^CCA
795 TGGCCA
MvaI 58 CCAGG CC^[AT]GG
192 CCTGG
SfaNI 293 GATGC GCATC(5/9)
498 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AluI BfaI BmyI BsiYI
BslI Bsp1286I BssKI BstF5I DpnII
EaeI FokI HaeIII HphI MaeI
MboI MboII MnlI MseI NdeII
NlaIII RsaI Sau3AI ScrFI Tru9I
Tsp509I