Restriction map for
pFN21AE6045
##################################
# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwNI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BanI BanII BbeI BbrPI BcgI
BclI BcnI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AciI 381 CCGC CCGC(-3/-1)
AflIII 317 ACATGT A^C[GA][CT]GT
Alw44I 85 GTGCAC G^TGCAC
AocI 465 CCTGAGG CC^T[ACGT]AGG
ApaLI 85 GTGCAC G^TGCAC
BamHI 674 GGATCC G^GATCC
BbsI 688 GTCTTC GAAGAC(2/6)
BbvCI 924 GCTGAGG CCTCAGC(-5/-2)
BciVI 65 GTATCC GTATCC(6/5)
BfaI 76 CTAG C^TAG
BglI 571 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 688 GTCTTC GAAGAC(2/6)
BseRI 468 GAGGAG GAGGAG(10/8)
BsmAI 179 GTCTC GTCTC(1/5)
Bsp1407I 476 TGTACA T^GTACA
BspLU11I 317 ACATGT A^CATGT
BsrGI 476 TGTACA T^GTACA
BstAPI 451 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 674 GGATCC [GA]^GATC[CT]
Bsu36I 465 CCTGAGG CC^T[ACGT]AGG
DsaI 368 CCATGG C^C[GA][CT]GG
EarI 404 CTCTTC CTCTTC(1/4)
Eco81I 465 CCTGAGG CC^T[ACGT]AGG
EcoNI 665 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 598 GTCAAC GT[CT]^[GA]AC
HindII 598 GTCAAC GT[CT]^[GA]AC
HphI 425 TCACC GGTGA(8/7)
Ksp632I 404 CTCTTC CTCTTC(1/4)
MaeI 76 CTAG C^TAG
MaeII 19 ACGT A^CGT
MaeIII 636 GTTAC ^GT[ACGT]AC
MflI 674 GGATCC [GA]^GATC[CT]
NcoI 368 CCATGG C^CATGG
NspI 317 ACATGT [GA]CATG^[CT]
SfcI 239 CTACAG C^T[GA][CT]AG
SmlI 937 CTCAAG C^T[CT][GA]AG
SspBI 476 TGTACA T^GTACA
XcmI 845 CCAACCTGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 674 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 15 GTCTAC GT^[AC][GT]AC
237 GTCTAC
AluI 711 AGCT AG^CT
723 AGCT
AspHI 85 GTGCAC G[AT]GC[AT]^C
556 GAGCAC
BbvI 305 GCTGC GCAGC(8/12)
513 GCTGC
BmyI 85 GTGCAC G[AGT]GC[ACT]^C
556 GAGCAC
BsiHKAI 85 GTGCAC G[AT]GC[AT]^C
556 GAGCAC
BsmFI 103 GTCCC GGGAC(10/14)
862 GTCCC
Bsp1286I 85 GTGCAC G[AGT]GC[ACT]^C
556 GAGCAC
BstXI 221 CCATTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
254 CCAAAATGTTGG
EcoT14I 368 CCATGG C^C[AT][AT]GG
615 CCTTGG
Fnu4HI 305 GCTGC GC^[ACGT]GC
513 GCTGC
ItaI 305 GCTGC GC^[ACGT]GC
513 GCTGC
MslI 339 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
745 CATATTGGTG
MwoI 451 GCAGGCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
571 GCCCGTCTGGC
NlaIV 470 GGAGCC GG[ACGT]^[ACGT]CC
674 GGATCC
StyI 368 CCATGG C^C[AT][AT]GG
615 CCTTGG
TaqI 714 TCGA T^CGA
912 TCGA
TfiI 217 GATTC G^A[AT]TC
909 GATTC
TseI 305 GCTGC G^C[AT]GC
513 GCTGC
TthHB8I 714 TCGA T^CGA
912 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AlwI BalI BsaJI BsiYI
BslI BsrDI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EaeI
EcoRII FokI HaeIII HinfI MboI
MboII MluNI MnlI MscI MvaI
NdeII NlaIII PleI RsaI Sau3AI
ScrFI SfaNI TspRI