Restriction map for
pFN21AE6068
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FauI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MaeI MaeII MboII MluI MroI
MspA1I MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 237 GGTACC G^GTACC
AciI 369 CCGC CCGC(-3/-1)
AcsI 922 AAATTT [GA]^AATT[CT]
AflIII 737 ACATGT A^C[GA][CT]GT
AhdI 544 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 16 AGCT AG^CT
Alw44I 225 GTGCAC G^TGCAC
AlwI 83 GGATC GGATC(4/5)
AlwNI 720 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 225 GTGCAC G^TGCAC
ApoI 922 AAATTT [GA]^AATT[CT]
Asp718I 237 GGTACC G^GTACC
AspEI 544 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 366 GGACC G^G[AT]CC
BanI 237 GGTACC G^G[CT][GA]CC
BanII 136 GGGCTC G[GA]GC[CT]^C
BbvI 766 GCTGC GCAGC(8/12)
BciVI 565 GTATCC GTATCC(6/5)
BclI 752 TGATCA T^GATCA
BcnI 513 CCGGG CC^[GC]GG
BglI 292 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 18 CTCCAG CTGGAG(16/14)
BsaBI 465 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 935 GAGAC GTCTC(1/5)
BsmFI 8 GGGAC GGGAC(10/14)
Bsp1407I 107 TGTACA T^GTACA
BspLU11I 737 ACATGT A^CATGT
BspMI 724 ACCTGC ACCTGC(4/8)
BsrDI 114 CATTGC GCAATG(2/0)
BsrFI 173 ACCGGC [GA]^CCGG[CT]
BsrGI 107 TGTACA T^GTACA
BstYI 83 GGATCT [GA]^GATC[CT]
CfoI 498 GCGC GCG^C
Cfr10I 173 ACCGGC [GA]^CCGG[CT]
DraII 164 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 219 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 233 CCATGG C^C[GA][CT]GG
Eam1105I 544 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 449 CCTTCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 164 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 634 GATATC GAT^ATC
FbaI 752 TGATCA T^GATCA
Fnu4HI 766 GCTGC GC^[ACGT]GC
HaeII 497 AGCGCC [GA]GCGC^[CT]
HhaI 498 GCGC GCG^C
HinP1I 498 GCGC G^CGC
ItaI 766 GCTGC GC^[ACGT]GC
KpnI 237 GGTACC GGTAC^C
MamI 465 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 875 CAATTG C^AATTG
MflI 83 GGATCT [GA]^GATC[CT]
MunI 875 CAATTG C^AATTG
NciI 513 CCGGG CC^[GC]GG
NcoI 233 CCATGG C^CATGG
NlaIV 237 GGTACC GG[ACGT]^[ACGT]CC
NspI 737 ACATGT [GA]CATG^[CT]
SspBI 107 TGTACA T^GTACA
TseI 766 GCTGC G^C[AT]GC
TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 83 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 225 GTGCAC G[AT]GC[AT]^C
346 GTGCTC
BseRI 487 CTCCTC GAGGAG(10/8)
160 GAGGAG
BsgI 169 CTGCAC GTGCAG(16/14)
726 CTGCAC
BsiHKAI 225 GTGCAC G[AT]GC[AT]^C
346 GTGCTC
DpnII 84 GATC ^GATC
753 GATC
HapII 174 CCGG C^CGG
513 CCGG
HpaII 174 CCGG C^CGG
513 CCGG
MboI 84 GATC ^GATC
753 GATC
MseI 287 TTAA T^TAA
920 TTAA
MspI 174 CCGG C^CGG
513 CCGG
NdeI 319 CATATG CA^TATG
547 CATATG
NdeII 84 GATC ^GATC
753 GATC
PleI 337 GAGTC GAGTC(4/5)
887 GAGTC
Sau3AI 84 GATC ^GATC
753 GATC
SfaNI 465 GATGC GCATC(5/9)
700 GATGC
SmlI 271 CTCAAG C^T[CT][GA]AG
453 CTTGAG
TfiI 35 GATTC G^A[AT]TC
903 GATTC
Tru9I 287 TTAA T^TAA
920 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI BalI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I EaeI EcoRII EcoT14I
FokI HaeIII HinfI HphI MaeIII
MluNI MnlI MscI MslI MvaI
MwoI NlaIII RsaI Sau96I ScrFI
StyI Tsp45I Tsp509I