Restriction map for
pFN21AE6059
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AgeI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaII
ItaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
TseI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 931 CTTAAG C^TTAAG
AflIII 548 ACATGT A^C[GA][CT]GT
AhdI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 83 GGATC GGATC(4/5)
AlwNI 720 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 711 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 366 GGACC G^G[AT]CC
BanI 16 GGCACC G^G[CT][GA]CC
BbsI 187 GTCTTC GAAGAC(2/6)
BfrI 931 CTTAAG C^TTAAG
BpmI 29 CTCCAG CTGGAG(16/14)
BpuAI 187 GTCTTC GAAGAC(2/6)
BsgI 726 CTGCAC GTGCAG(16/14)
BsmFI 801 GGGAC GGGAC(10/14)
Bsp1407I 107 TGTACA T^GTACA
BspLU11I 548 ACATGT A^CATGT
BsrGI 107 TGTACA T^GTACA
BstYI 83 GGATCT [GA]^GATC[CT]
Cac8I 292 GCCTGC GC[ACGT]^[ACGT]GC
DdeI 454 CTTAG C^T[ACGT]AG
DpnII 84 GATC ^GATC
DraI 286 TTTAAA TTT^AAA
Eam1105I 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 250 CTCTTC CTCTTC(1/4)
HincII 586 GTTAAC GT[CT]^[GA]AC
HindII 586 GTTAAC GT[CT]^[GA]AC
HpaI 586 GTTAAC GTT^AAC
Ksp632I 250 CTCTTC CTCTTC(1/4)
MaeII 521 ACGT A^CGT
MaeIII 542 GTGAC ^GT[ACGT]AC
MboI 84 GATC ^GATC
MflI 83 GGATCT [GA]^GATC[CT]
MwoI 292 GCCTGCCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 84 GATC ^GATC
NspI 548 ACATGT [GA]CATG^[CT]
PflMI 369 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI 84 GATC ^GATC
SspBI 107 TGTACA T^GTACA
TaqI 705 TCGA T^CGA
TfiI 666 GATTC G^A[AT]TC
Tsp45I 542 GTGAC ^GT[GC]AC
TthHB8I 705 TCGA T^CGA
Van91I 369 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 83 GGATCT [GA]^GATC[CT]
XmnI 711 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 33 AGCT AG^CT
435 AGCT
Alw44I 225 GTGCAC G^TGCAC
316 GTGCAC
ApaLI 225 GTGCAC G^TGCAC
316 GTGCAC
BalI 377 TGGCCA TGG^CCA
557 TGGCCA
BsaJI 57 CCCTGG C^C[ACGT][ACGT]GG
362 CCCTGG
BspMI 724 ACCTGC ACCTGC(4/8)
900 GCAGGT
BsrDI 114 CATTGC GCAATG(2/0)
426 CATTGC
Cfr13I 360 GGCCC G^G[ACGT]CC
366 GGACC
DraIII 370 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
492 CACCAAGTG
EaeI 377 TGGCCA [CT]^GGCC[GA]
557 TGGCCA
EcoRV 564 GATATC GAT^ATC
634 GATATC
MluNI 377 TGGCCA TGG^CCA
557 TGGCCA
MscI 377 TGGCCA TGG^CCA
557 TGGCCA
NlaIV 16 GGCACC GG[ACGT]^[ACGT]CC
440 GGTTCC
PleI 337 GAGTC GAGTC(4/5)
684 GAGTC
Sau96I 360 GGCCC G^G[ACGT]CC
366 GGACC
SmlI 271 CTCAAG C^T[CT][GA]AG
931 CTTAAG
StuI 164 AGGCCT AGG^CCT
713 AGGCCT
Tsp509I 418 AATT ^AATT
946 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AspHI BmyI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
EcoRII FokI HaeIII HinfI HphI
MboII MnlI MseI MslI MvaI
NlaIII RsaI ScrFI SfaNI Tru9I
TspRI