Restriction map for
pFN21AE6082
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BpmI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I StuI SwaI TaqI
Tth111I TthHB8I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 390 CCGC CCGC(-3/-1)
AhdI 565 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 741 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 474 CCTGAGG CC^T[ACGT]AGG
Asp700I 732 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 565 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 367 GTGCTC G[AT]GC[AT]^C
BalI 213 TGGCCA TGG^CCA
BbvI 697 GCAGC GCAGC(8/12)
BfaI 385 CTAG C^TAG
BlpI 457 GCTGAGC GC^T[ACGT]AGC
BmyI 367 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 457 GCTGAGC GC^T[ACGT]AGC
BsaAI 555 TACGTA [CT]AC^GT[GA]
BsiHKAI 367 GTGCTC G[AT]GC[AT]^C
BsmAI 290 GTCTC GTCTC(1/5)
BsmFI 822 GGGAC GGGAC(10/14)
BsmI 790 GCATTC GAATGC(1/-1)
Bsp1286I 367 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 125 TGTACA T^GTACA
BsrDI 447 CATTGC GCAATG(2/0)
BsrGI 125 TGTACA T^GTACA
BstF5I 193 CATCC GGATG(2/0)
BstXI 810 CCACCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 101 GGATCT [GA]^GATC[CT]
Bsu36I 474 CCTGAGG CC^T[ACGT]AGG
CelII 457 GCTGAGC GC^T[ACGT]AGC
Cfr13I 321 GGCCC G^G[ACGT]CC
EaeI 213 TGGCCA [CT]^GGCC[GA]
Eam1105I 565 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 474 CCTGAGG CC^T[ACGT]AGG
EcoNI 470 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 452 CCAAGG C^C[AT][AT]GG
Fnu4HI 697 GCAGC GC^[ACGT]GC
FokI 193 CATCC GGATG(9/13)
ItaI 697 GCAGC GC^[ACGT]GC
MaeI 385 CTAG C^TAG
MaeII 556 ACGT A^CGT
MaeIII 43 GTGAC ^GT[ACGT]AC
MflI 101 GGATCT [GA]^GATC[CT]
MluNI 213 TGGCCA TGG^CCA
MscI 213 TGGCCA TGG^CCA
Sau96I 321 GGCCC G^G[ACGT]CC
SexAI 75 ACCTGGT A^CC[AT]GGT
SfcI 575 CTGTAG C^T[GA][CT]AG
SmlI 691 CTCAAG C^T[CT][GA]AG
SnaBI 555 TACGTA TAC^GTA
SspBI 125 TGTACA T^GTACA
SspI 541 AATATT AAT^ATT
StyI 452 CCAAGG C^C[AT][AT]GG
TseI 697 GCAGC G^C[AT]GC
Tsp45I 43 GTGAC ^GT[GC]AC
TspRI 750 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 384 TCTAGA T^CTAGA
XhoII 101 GGATCT [GA]^GATC[CT]
XmnI 732 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AflIII 93 ACATGT A^C[GA][CT]GT
569 ACATGT
AluI 461 AGCT AG^CT
584 AGCT
AlwI 687 GATCC GGATC(4/5)
101 GGATC
BsaJI 452 CCAAGG C^C[ACGT][ACGT]GG
951 CCAGGG
BseRI 47 CTCCTC GAGGAG(10/8)
178 GAGGAG
BspLU11I 93 ACATGT A^CATGT
569 ACATGT
BssKI 76 CCTGG ^CC[ACGT]GG
951 CCAGG
BstNI 76 CCTGG CC^[AT]GG
951 CCAGG
DdeI 458 CTGAG C^T[ACGT]AG
475 CTGAG
EarI 711 CTCTTC CTCTTC(1/4)
842 CTCTTC
EcoRII 76 CCTGG ^CC[AT]GG
951 CCAGG
HaeIII 214 GGCC GG^CC
321 GGCC
Ksp632I 711 CTCTTC CTCTTC(1/4)
842 CTCTTC
MvaI 76 CCTGG CC^[AT]GG
951 CCAGG
MwoI 313 GCTTGCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
457 GCTGAGCTTGC
NspI 93 ACATGT [GA]CATG^[CT]
569 ACATGT
ScrFI 76 CCTGG CC^[ACGT]GG
951 CCAGG
TfiI 147 GAATC G^A[AT]TC
495 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI BsgI BsiYI BslI BspMI
Cac8I DpnII HinfI HphI MboI
MboII MnlI MseI MslI NdeII
NlaIII PleI RsaI Sau3AI SfaNI
Tru9I Tsp509I