Restriction map for
pFN21AE6083
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 232 GTAGAC GT^[AC][GT]AC
AcsI 43 AAATTC [GA]^AATT[CT]
AlwI 267 GATCC GGATC(4/5)
AlwNI 500 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 314 AGCGCT AGC^GCT
ApoI 43 AAATTC [GA]^AATT[CT]
AvaI 78 CCCGGG C^[CT]CG[GA]G
BciVI 365 GTATCC GTATCC(6/5)
BglII 967 AGATCT A^GATCT
BpmI 497 CTCCAG CTGGAG(16/14)
BsaI 51 GGTCTC GGTCTC(1/5)
BseRI 370 CTCCTC GAGGAG(10/8)
BsmAI 52 GTCTC GTCTC(1/5)
BsmI 83 GCATTC GAATGC(1/-1)
BsoBI 78 CCCGGG C^[CT]CG[GA]G
BstAPI 139 GCACTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 315 GCGC GCG^C
Cfr13I 735 GGCCC G^G[ACGT]CC
DraII 734 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 518 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 413 CTCTTC CTCTTC(1/4)
Eco47III 314 AGCGCT AGC^GCT
EcoO109I 734 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 314 AGCGCT [GA]GCGC^[CT]
HapII 79 CCGG C^CGG
HhaI 315 GCGC GCG^C
HinP1I 315 GCGC G^CGC
HpaII 79 CCGG C^CGG
Ksp632I 413 CTCTTC CTCTTC(1/4)
MfeI 428 CAATTG C^AATTG
MslI 495 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 79 CCGG C^CGG
MunI 428 CAATTG C^AATTG
NdeI 7 CATATG CA^TATG
PflMI 275 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 235 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 725 CTGCAG CTGCA^G
Sau96I 735 GGCCC G^G[ACGT]CC
SmaI 78 CCCGGG CCC^GGG
Van91I 275 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 78 CCCGGG C^CCGGG
XmaI 78 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 693 GTAC GT^AC
700 GTAC
AspHI 561 GTGCTC G[AT]GC[AT]^C
741 GAGCAC
BalI 245 TGGCCA TGG^CCA
272 TGGCCA
BcnI 78 CCCGG CC^[GC]GG
79 CCGGG
BmyI 561 GTGCTC G[AGT]GC[ACT]^C
741 GAGCAC
BsiHKAI 561 GTGCTC G[AT]GC[AT]^C
741 GAGCAC
Bsp1286I 561 GTGCTC G[AGT]GC[ACT]^C
741 GAGCAC
BstYI 266 AGATCC [GA]^GATC[CT]
967 AGATCT
Cac8I 92 GCTGGC GC[ACGT]^[ACGT]GC
510 GCTTGC
EaeI 245 TGGCCA [CT]^GGCC[GA]
272 TGGCCA
MboII 278 TCTTC GAAGA(8/7)
414 TCTTC
MflI 266 AGATCC [GA]^GATC[CT]
967 AGATCT
MluNI 245 TGGCCA TGG^CCA
272 TGGCCA
MscI 245 TGGCCA TGG^CCA
272 TGGCCA
MspA1I 90 CAGCTG C[AC]G^C[GT]G
716 CAGCTG
NciI 78 CCCGG CC^[GC]GG
79 CCGGG
PleI 807 GACTC GAGTC(4/5)
358 GAGTC
PvuII 90 CAGCTG CAG^CTG
716 CAGCTG
RsaI 693 GTAC GT^AC
700 GTAC
SfcI 725 CTGCAG C^T[GA][CT]AG
890 CTACAG
XhoII 266 AGATCC [GA]^GATC[CT]
967 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MnlI MseI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI