Restriction map for
pFN21AE3966
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 962 GAATTT [GA]^AATT[CT]
AcyI 754 GGCGTC G[GA]^CG[CT]C
AfaI 411 GTAC GT^AC
AflIII 380 ACACGT A^C[GA][CT]GT
Alw44I 284 GTGCAC G^TGCAC
AlwI 176 GGATC GGATC(4/5)
ApaLI 284 GTGCAC G^TGCAC
ApoI 962 GAATTT [GA]^AATT[CT]
AspI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 404 CCCGGG C^[CT]CG[GA]G
BbvCI 444 GCTGAGG CCTCAGC(-5/-2)
BclI 276 TGATCA T^GATCA
BglI 204 GCCTAATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 699 AGATCT A^GATCT
BsaHI 754 GGCGTC G[GA]^CG[CT]C
BsaI 356 GGTCTC GGTCTC(1/5)
BsgI 664 GTGCAG GTGCAG(16/14)
BsmFI 678 GTCCC GGGAC(10/14)
BsmI 848 GAATGC GAATGC(1/-1)
BsoBI 404 CCCGGG C^[CT]CG[GA]G
BspMI 40 ACCTGC ACCTGC(4/8)
BsrBI 352 GAGCGG CCGCTC(-3/-3)
BsrDI 590 GCAATG GCAATG(2/0)
BstAPI 708 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 699 AGATCT [GA]^GATC[CT]
DrdI 325 GACGGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 90 CGGCCA [CT]^GGCC[GA]
EcoT14I 291 CCATGG C^C[AT][AT]GG
FbaI 276 TGATCA T^GATCA
Hin1I 754 GGCGTC G[GA]^CG[CT]C
HindIII 500 AAGCTT A^AGCTT
MflI 699 AGATCT [GA]^GATC[CT]
NcoI 291 CCATGG C^CATGG
PflFI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 513 GACTC GAGTC(4/5)
PshAI 873 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 715 CTGCAG CTGCA^G
RsaI 411 GTAC GT^AC
SfcI 715 CTGCAG C^T[GA][CT]AG
SmaI 404 CCCGGG CCC^GGG
SphI 109 GCATGC GCATG^C
StyI 291 CCATGG C^C[AT][AT]GG
TaqI 828 TCGA T^CGA
Tsp509I 963 AATT ^AATT
Tth111I 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 828 TCGA T^CGA
XhoII 699 AGATCT [GA]^GATC[CT]
XmaCI 404 CCCGGG C^CCGGG
XmaI 404 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 96 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
676 CAGTCCCTG
Asp700I 622 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
796 GAAACTTTTC
BbsI 510 GAAGAC GAAGAC(2/6)
622 GAAGAC
BcnI 404 CCCGG CC^[GC]GG
405 CCGGG
BfaI 142 CTAG C^TAG
853 CTAG
BpuAI 510 GAAGAC GAAGAC(2/6)
622 GAAGAC
BsmAI 566 GAGAC GTCTC(1/5)
357 GTCTC
BstEII 36 GGTGACC G^GT[ACGT]ACC
373 GGTCACC
BstF5I 570 CATCC GGATG(2/0)
750 GGATG
BstPI 36 GGTGACC G^GT[ACGT]ACC
373 GGTCACC
DsaI 291 CCATGG C^C[GA][CT]GG
369 CCGTGG
EcoNI 387 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
606 CCTGGAGAAGG
EcoO65I 36 GGTGACC G^GT[ACGT]ACC
373 GGTCACC
FokI 570 CATCC GGATG(9/13)
750 GGATG
HaeIII 91 GGCC GG^CC
203 GGCC
HapII 174 CCGG C^CGG
405 CCGG
HgaI 884 GACGC GACGC(5/10)
755 GCGTC
HpaII 174 CCGG C^CGG
405 CCGG
HphI 36 GGTGA GGTGA(8/7)
375 TCACC
MaeI 142 CTAG C^TAG
853 CTAG
MseI 20 TTAA T^TAA
992 TTAA
MspI 174 CCGG C^CGG
405 CCGG
NciI 404 CCCGG CC^[GC]GG
405 CCGGG
NspI 109 GCATGC [GA]CATG^[CT]
213 GCATGT
SacI 688 GAGCTC GAGCT^C
830 GAGCTC
SfaNI 441 GATGC GCATC(5/9)
708 GCATC
TfiI 11 GAATC G^A[AT]TC
1003 GATTC
Tru9I 20 TTAA T^TAA
992 TTAA
TspRI 117 CACTG CA[GC]TG[ACGT][ACGT]^
725 CAGTG
XmnI 622 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
796 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI BstXI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI HinfI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI TseI Tsp45I