Restriction map for
pFN21AE6062
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BanI BbeI BbrPI BbvCI BbvI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII HphI ItaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MseI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I TseI Tsp45I TspRI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 292 CGCG CG^CG
AciI 723 CCGC CCGC(-3/-1)
AcsI 107 GAATTC [GA]^AATT[CT]
AfaI 953 GTAC GT^AC
AhdI 818 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 107 GAATTC [GA]^AATT[CT]
AspEI 818 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 52 GGACC G^G[AT]CC
BamHI 71 GGATCC G^GATCC
BanII 287 GGGCTC G[GA]GC[CT]^C
BcgI 475 CGAAGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 20 TGATCA T^GATCA
BfaI 283 CTAG C^TAG
BmyI 287 GGGCTC G[AGT]GC[ACT]^C
BpmI 82 CTGGAG CTGGAG(16/14)
BsgI 878 CTGCAC GTGCAG(16/14)
BsmAI 826 GTCTC GTCTC(1/5)
Bsp1286I 287 GGGCTC G[AGT]GC[ACT]^C
BsrDI 663 CATTGC GCAATG(2/0)
BstBI 473 TTCGAA TT^CGAA
BstUI 292 CGCG CG^CG
BstYI 71 GGATCC [GA]^GATC[CT]
Cfr13I 52 GGACC G^G[ACGT]CC
DraII 51 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 818 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 51 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 107 GAATTC G^AATTC
EcoRV 595 GATATC GAT^ATC
FauI 722 CCCGC CCCGC(4/6)
FbaI 20 TGATCA T^GATCA
HindIII 477 AAGCTT A^AGCTT
MaeI 283 CTAG C^TAG
MaeIII 41 GTTAC ^GT[ACGT]AC
MflI 71 GGATCC [GA]^GATC[CT]
MspA1I 248 CAGCTG C[AC]G^C[GT]G
MvnI 292 CGCG CG^CG
NruI 291 TCGCGA TCG^CGA
NspI 514 ACATGC [GA]CATG^[CT]
NspV 473 TTCGAA TT^CGAA
PleI 432 GACTC GAGTC(4/5)
PpuMI 51 AGGACCC [GA]G^G[AT]CC[CT]
PstI 528 CTGCAG CTGCA^G
PvuII 248 CAGCTG CAG^CTG
RsaI 953 GTAC GT^AC
Sau96I 52 GGACC G^G[ACGT]CC
SfcI 528 CTGCAG C^T[GA][CT]AG
SfuI 473 TTCGAA TT^CGAA
SmlI 411 CTTGAG C^T[CT][GA]AG
StuI 78 AGGCCT AGG^CCT
TaqI 474 TCGA T^CGA
TthHB8I 474 TCGA T^CGA
XcmI 93 CCATGTGTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 71 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 72 GATCC GGATC(4/5)
71 GGATC
BalI 221 TGGCCA TGG^CCA
371 TGGCCA
BbsI 906 GAAGAC GAAGAC(2/6)
185 GTCTTC
BpuAI 906 GAAGAC GAAGAC(2/6)
185 GTCTTC
BsiYI 75 CCCAGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
165 CCTCATTGTGG
BslI 75 CCCAGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
165 CCTCATTGTGG
BspHI 189 TCATGA T^CATGA
635 TCATGA
BspMI 783 ACCTGC ACCTGC(4/8)
876 ACCTGC
BstXI 362 CCATTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
807 CCATCGCTTTGG
DsaI 374 CCATGG C^C[GA][CT]GG
890 CCATGG
EaeI 221 TGGCCA [CT]^GGCC[GA]
371 TGGCCA
EcoT14I 374 CCATGG C^C[AT][AT]GG
890 CCATGG
MluNI 221 TGGCCA TGG^CCA
371 TGGCCA
MscI 221 TGGCCA TGG^CCA
371 TGGCCA
MwoI 530 GCAGGACACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
577 GCCCAGCTCGC
NcoI 374 CCATGG C^CATGG
890 CCATGG
NlaIV 52 GGACCC GG[ACGT]^[ACGT]CC
71 GGATCC
RcaI 189 TCATGA T^CATGA
635 TCATGA
StyI 374 CCATGG C^C[AT][AT]GG
890 CCATGG
TfiI 355 GATTC G^A[AT]TC
657 GATTC
Tsp509I 89 AATT ^AATT
108 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BsaJI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII FokI
HaeIII HinfI MboI MboII MnlI
MslI MvaI NdeII NlaIII Sau3AI
ScrFI SfaNI