Restriction map for
pFN21AE5962
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FokI FseI
FspI HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Alw44I 781 GTGCAC G^TGCAC
ApaI 85 GGGCCC GGGCC^C
ApaLI 781 GTGCAC G^TGCAC
AvaII 627 GGACC G^G[AT]CC
BcgI 247 CGAACCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 58 CTGGAG CTGGAG(16/14)
BsaI 771 GGTCTC GGTCTC(1/5)
BsmAI 772 GTCTC GTCTC(1/5)
BsmFI 802 GTCCC GGGAC(10/14)
BsmI 28 GCATTC GAATGC(1/-1)
BsrI 43 ACTGG ACTGG(1/-1)
BstAPI 138 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 215 CCATATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 508 AGATCC [GA]^GATC[CT]
DsaI 221 CCATGG C^C[GA][CT]GG
EarI 473 CTCTTC CTCTTC(1/4)
EcoRV 571 GATATC GAT^ATC
FauI 266 CCCGC CCCGC(4/6)
HaeII 877 AGCGCC [GA]GCGC^[CT]
HindIII 453 AAGCTT A^AGCTT
Ksp632I 473 CTCTTC CTCTTC(1/4)
MflI 508 AGATCC [GA]^GATC[CT]
MseI 488 TTAA T^TAA
MspA1I 443 CAGCTG C[AC]G^C[GT]G
NcoI 221 CCATGG C^CATGG
PflMI 760 CCAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 596 GACTC GAGTC(4/5)
PvuII 443 CAGCTG CAG^CTG
SacI 63 GAGCTC GAGCT^C
SbfI 135 CCTGCAGG CCTGCA^GG
Sse8387I 135 CCTGCAGG CCTGCA^GG
TaqI 450 TCGA T^CGA
TfiI 331 GAATC G^A[AT]TC
Tru9I 488 TTAA T^TAA
Tsp509I 294 AATT ^AATT
TthHB8I 450 TCGA T^CGA
Van91I 760 CCAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 614 CCACCTTTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 508 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 718 ACATGT A^C[GA][CT]GT
816 ACATGT
AlwI 132 GATCC GGATC(4/5)
509 GATCC
BciVI 47 GTATCC GTATCC(6/5)
610 GTATCC
BsgI 538 CTGCAC GTGCAG(16/14)
440 GTGCAG
BspLU11I 718 ACATGT A^CATGT
816 ACATGT
BsrDI 420 CATTGC GCAATG(2/0)
427 GCAATG
CfoI 390 GCGC GCG^C
878 GCGC
DpnII 132 GATC ^GATC
509 GATC
DraII 84 AGGGCCC [GA]G^G[ACGT]CC[CT]
85 GGGCCCC
EcoO109I 84 AGGGCCC [GA]G^G[ACGT]CC[CT]
85 GGGCCCC
EcoT14I 114 CCTTGG C^C[AT][AT]GG
221 CCATGG
HhaI 390 GCGC GCG^C
878 GCGC
HinP1I 390 GCGC G^CGC
878 GCGC
HinfI 331 GAATC G^A[ACGT]TC
596 GACTC
MboI 132 GATC ^GATC
509 GATC
MslI 216 CATATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
717 CACATGTGTG
NdeII 132 GATC ^GATC
509 GATC
PstI 136 CTGCAG CTGCA^G
504 CTGCAG
Sau3AI 132 GATC ^GATC
509 GATC
SfaNI 283 GATGC GCATC(5/9)
587 GCATC
SfcI 136 CTGCAG C^T[GA][CT]AG
504 CTGCAG
StyI 114 CCTTGG C^C[AT][AT]GG
221 CCATGG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AspHI BalI
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I Cfr13I EaeI EcoRII Fnu4HI
HaeIII HphI ItaI MaeIII MboII
MluNI MnlI MscI MvaI MwoI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI TseI Tsp45I TspRI