Restriction map for
pFN21AE6036
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpmI BsaAI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 268 AACGTT AA^CGTT
AcsI 918 AAATTT [GA]^AATT[CT]
ApoI 918 AAATTT [GA]^AATT[CT]
BamHI 633 GGATCC G^GATCC
BanI 852 GGCACC G^G[CT][GA]CC
BbsI 339 GTCTTC GAAGAC(2/6)
BcnI 858 CCCGG CC^[GC]GG
BfaI 742 CTAG C^TAG
BglI 691 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 700 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 700 GCTGAGC GC^T[ACGT]AGC
BpuAI 339 GTCTTC GAAGAC(2/6)
BsaBI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 220 GGTCTC GGTCTC(1/5)
BsmI 942 GCATTC GAATGC(1/-1)
BspMI 845 ACCTGC ACCTGC(4/8)
CelII 700 GCTGAGC GC^T[ACGT]AGC
CfoI 177 GCGC GCG^C
DsaI 767 CCATGG C^C[GA][CT]GG
EcoNI 543 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 767 CCATGG C^C[AT][AT]GG
EcoT22I 940 ATGCAT ATGCA^T
HapII 859 CCGG C^CGG
HhaI 177 GCGC GCG^C
HinP1I 177 GCGC G^CGC
HincII 364 GTTGAC GT[CT]^[GA]AC
HindII 364 GTTGAC GT[CT]^[GA]AC
HpaII 859 CCGG C^CGG
MaeI 742 CTAG C^TAG
MaeII 269 ACGT A^CGT
MaeIII 10 GTGAC ^GT[ACGT]AC
MamI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 916 TTAA T^TAA
MspA1I 591 CAGCTG C[AC]G^C[GT]G
MspI 859 CCGG C^CGG
NciI 858 CCCGG CC^[GC]GG
NcoI 767 CCATGG C^CATGG
NsiI 940 ATGCAT ATGCA^T
PshAI 214 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 268 AACGTT AA^CGTT
PvuII 591 CAGCTG CAG^CTG
SfaNI 32 GCATC GCATC(5/9)
SmlI 493 CTCAAG C^T[CT][GA]AG
SphI 804 GCATGC GCATG^C
StyI 767 CCATGG C^C[AT][AT]GG
Tru9I 916 TTAA T^TAA
Tsp45I 10 GTGAC ^GT[GC]AC
Tsp509I 919 AATT ^AATT
XcmI 113 CCATCTCCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 561 CCGC CCGC(-3/-1)
469 GCGG
BalI 377 TGGCCA TGG^CCA
833 TGGCCA
BbvI 252 GCAGC GCAGC(8/12)
455 GCTGC
BmyI 684 GGGCAC G[AGT]GC[ACT]^C
713 GTGCCC
BsmAI 104 GTCTC GTCTC(1/5)
221 GTCTC
Bsp1286I 684 GGGCAC G[AGT]GC[ACT]^C
713 GTGCCC
BsrI 436 ACTGG ACTGG(1/-1)
816 ACTGG
EaeI 377 TGGCCA [CT]^GGCC[GA]
833 TGGCCA
Fnu4HI 252 GCAGC GC^[ACGT]GC
455 GCTGC
ItaI 252 GCAGC GC^[ACGT]GC
455 GCTGC
MluNI 377 TGGCCA TGG^CCA
833 TGGCCA
MscI 377 TGGCCA TGG^CCA
833 TGGCCA
MslI 243 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
762 CATTCCCATG
NlaIV 633 GGATCC GG[ACGT]^[ACGT]CC
852 GGCACC
NspI 179 GCATGT [GA]CATG^[CT]
804 GCATGC
StuI 68 AGGCCT AGG^CCT
290 AGGCCT
TfiI 278 GAATC G^A[AT]TC
323 GATTC
TseI 252 GCAGC G^C[AT]GC
455 GCTGC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
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AluI AlwI AvaII BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII FokI HaeIII HinfI HphI
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII PleI Sau3AI
Sau96I ScrFI TspRI XhoII