Restriction map for
pFN21AE5427
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 171 GTAC GT^AC
AflIII 517 ACACGT A^C[GA][CT]GT
AluI 60 AGCT AG^CT
AlwI 71 GGATC GGATC(4/5)
BanII 163 GAGCCC G[GA]GC[CT]^C
BbrPI 518 CACGTG CAC^GTG
BbsI 155 GTCTTC GAAGAC(2/6)
BciVI 454 GTATCC GTATCC(6/5)
BclI 629 TGATCA T^GATCA
BmyI 163 GAGCCC G[AGT]GC[ACT]^C
BpmI 52 CTGGAG CTGGAG(16/14)
BpuAI 155 GTCTTC GAAGAC(2/6)
BsaAI 518 CACGTG [CT]AC^GT[GA]
BsaBI 72 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 46 CCAGGG C^C[ACGT][ACGT]GG
BseRI 127 CTCCTC GAGGAG(10/8)
BsiYI 339 CCTGGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 339 CCTGGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 549 GAGAC GTCTC(1/5)
Bsp1286I 163 GAGCCC G[AGT]GC[ACT]^C
BstYI 71 GGATCT [GA]^GATC[CT]
Cfr13I 5 GGCCC G^G[ACGT]CC
DdeI 442 CTAAG C^T[ACGT]AG
FbaI 629 TGATCA T^GATCA
HaeIII 5 GGCC GG^CC
HgaI 477 GCGTC GACGC(5/10)
MaeII 519 ACGT A^CGT
MamI 72 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 71 GGATCT [GA]^GATC[CT]
MslI 813 CATTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 350 CATATG CA^TATG
NlaIV 331 GGTTCC GG[ACGT]^[ACGT]CC
NspI 122 GCATGC [GA]CATG^[CT]
PmaCI 518 CACGTG CAC^GTG
PmlI 518 CACGTG CAC^GTG
PshAI 148 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 17 CTGCAG CTGCA^G
RsaI 171 GTAC GT^AC
Sau96I 5 GGCCC G^G[ACGT]CC
SphI 122 GCATGC GCATG^C
SspI 10 AATATT AAT^ATT
Tsp45I 211 GTGAC ^GT[GC]AC
XhoII 71 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 372 CCGC CCGC(-3/-1)
906 CCGC
BfaI 410 CTAG C^TAG
619 CTAG
BssKI 46 CCAGG ^CC[ACGT]GG
339 CCTGG
BstNI 46 CCAGG CC^[AT]GG
339 CCTGG
EarI 637 CTCTTC CTCTTC(1/4)
688 GAAGAG
EcoRII 46 CCAGG ^CC[AT]GG
339 CCTGG
Ksp632I 637 CTCTTC CTCTTC(1/4)
688 GAAGAG
MaeI 410 CTAG C^TAG
619 CTAG
MvaI 46 CCAGG CC^[AT]GG
339 CCTGG
MwoI 272 GCAGTGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
899 GCATTATCCGC
PleI 622 GACTC GAGTC(4/5)
413 GAGTC
ScrFI 46 CCAGG CC^[ACGT]GG
339 CCTGG
TfiI 255 GAATC G^A[AT]TC
325 GAATC
TspRI 67 CACTG CA[GC]TG[ACGT][ACGT]^
273 CAGTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
BbvI BspHI BsrDI BsrI BstF5I
Cac8I DpnII Fnu4HI FokI HinfI
HphI ItaI MaeIII MboI MboII
MnlI MseI NdeII NlaIII RcaI
Sau3AI SfaNI SfcI Tru9I TseI
Tsp509I