Restriction map for
pFN21AE6090
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BanII BbeI
BbrPI BcgI BciVI BcnI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 810 CCGC CCGC(-3/-1)
Asp700I 67 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 544 GAGCAC G[AT]GC[AT]^C
BamHI 53 GGATCC G^GATCC
BanI 849 GGTGCC G^G[CT][GA]CC
BbsI 683 GTCTTC GAAGAC(2/6)
BbvCI 714 CCTCAGC CCTCAGC(-5/-2)
BbvI 164 GCAGC GCAGC(8/12)
BclI 149 TGATCA T^GATCA
BglI 196 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 896 AGATCT A^GATCT
BpuAI 683 GTCTTC GAAGAC(2/6)
BsaI 220 GGTCTC GGTCTC(1/5)
BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG
BseRI 33 CTCCTC GAGGAG(10/8)
BsiHKAI 544 GAGCAC G[AT]GC[AT]^C
BsmAI 221 GTCTC GTCTC(1/5)
BsmFI 711 GTCCC GGGAC(10/14)
Bsp1407I 107 TGTACA T^GTACA
BspHI 693 TCATGA T^CATGA
BsrGI 107 TGTACA T^GTACA
BsrI 21 CCAGT ACTGG(1/-1)
BstXI 209 CCACTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 404 GCGC GCG^C
DrdI 631 GACATCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 949 GAAGAG CTCTTC(1/4)
FauI 809 CCCGC CCCGC(4/6)
FbaI 149 TGATCA T^GATCA
Fnu4HI 164 GCAGC GC^[ACGT]GC
HaeII 403 AGCGCC [GA]GCGC^[CT]
HhaI 404 GCGC GCG^C
HinP1I 404 GCGC G^CGC
ItaI 164 GCAGC GC^[ACGT]GC
Ksp632I 949 GAAGAG CTCTTC(1/4)
MseI 463 TTAA T^TAA
PshAI 704 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 693 TCATGA T^CATGA
SfiI 195 GGCCATGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 706 CTCAAG C^T[CT][GA]AG
SspBI 107 TGTACA T^GTACA
Tru9I 463 TTAA T^TAA
TseI 164 GCAGC G^C[AT]GC
TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 206 CCACCACTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 67 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 108 GTAC GT^AC
954 GTAC
AflIII 721 ACATGT A^C[GA][CT]GT
732 ACATGT
AluI 231 AGCT AG^CT
370 AGCT
BmyI 544 GAGCAC G[AGT]GC[ACT]^C
805 GTGCCC
Bsp1286I 544 GAGCAC G[AGT]GC[ACT]^C
805 GTGCCC
BspLU11I 721 ACATGT A^CATGT
732 ACATGT
BsrDI 642 CATTGC GCAATG(2/0)
355 GCAATG
BssKI 58 CCAGG ^CC[ACGT]GG
192 CCTGG
BstF5I 237 CATCC GGATG(2/0)
408 CATCC
BstNI 58 CCAGG CC^[AT]GG
192 CCTGG
BstYI 53 GGATCC [GA]^GATC[CT]
896 AGATCT
Cac8I 292 GCCTGC GC[ACGT]^[ACGT]GC
574 GCCAGC
EcoRII 58 CCAGG ^CC[AT]GG
192 CCTGG
FokI 237 CATCC GGATG(9/13)
408 CATCC
MflI 53 GGATCC [GA]^GATC[CT]
896 AGATCT
MvaI 58 CCAGG CC^[AT]GG
192 CCTGG
MwoI 196 GCCATGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
852 GCCACCAATGC
NlaIV 53 GGATCC GG[ACGT]^[ACGT]CC
849 GGTGCC
NspI 721 ACATGT [GA]CATG^[CT]
732 ACATGT
RsaI 108 GTAC GT^AC
954 GTAC
ScrFI 58 CCAGG CC^[ACGT]GG
192 CCTGG
SfaNI 246 GATGC GCATC(5/9)
578 GCATC
SspI 589 AATATT AAT^ATT
918 AATATT
TfiI 254 GAATC G^A[AT]TC
516 GAATC
Tsp509I 465 AATT ^AATT
525 AATT
XhoII 53 GGATCC [GA]^GATC[CT]
896 AGATCT
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AlwI BalI BfaI BsiYI BslI
DdeI DpnII EaeI HaeIII HinfI
HphI MaeI MboI MboII MluNI
MnlI MscI MslI NdeII NlaIII
PleI Sau3AI