Restriction map for
pFN21AE5935
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbvI BcgI BciVI
BclI BfrI BglI BglII BlnI
BpmI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI ItaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TseI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AvaII 887 GGACC G^G[AT]CC
BalI 194 TGGCCA TGG^CCA
BanII 175 GAGCCC G[GA]GC[CT]^C
BbrPI 720 CACGTG CAC^GTG
BbvCI 340 GCTGAGG CCTCAGC(-5/-2)
BfaI 145 CTAG C^TAG
BlpI 700 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 700 GCTCAGC GC^T[ACGT]AGC
BsaAI 720 CACGTG [CT]AC^GT[GA]
BsmFI 458 GTCCC GGGAC(10/14)
BspDI 788 ATCGAT AT^CGAT
BspLU11I 77 ACATGT A^CATGT
BssSI 274 CACGAG CACGAG(-5/-1)
BstF5I 416 CATCC GGATG(2/0)
BstYI 83 GGATCT [GA]^GATC[CT]
Cac8I 245 GCATGC GC[ACGT]^[ACGT]GC
CelII 700 GCTCAGC GC^T[ACGT]AGC
CfoI 728 GCGC GCG^C
ClaI 788 ATCGAT AT^CGAT
DsaI 479 CCATGG C^C[GA][CT]GG
EcoT14I 479 CCATGG C^C[AT][AT]GG
FokI 416 CATCC GGATG(9/13)
HaeII 727 GGCGCT [GA]GCGC^[CT]
HhaI 728 GCGC GCG^C
HinP1I 728 GCGC G^CGC
MaeI 145 CTAG C^TAG
MaeII 721 ACGT A^CGT
MflI 83 GGATCT [GA]^GATC[CT]
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
MseI 281 TTAA T^TAA
NcoI 479 CCATGG C^CATGG
NlaIV 174 GGAGCC GG[ACGT]^[ACGT]CC
PflMI 420 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 720 CACGTG CAC^GTG
PmlI 720 CACGTG CAC^GTG
SphI 245 GCATGC GCATG^C
StyI 479 CCATGG C^C[AT][AT]GG
Tru9I 281 TTAA T^TAA
Tsp45I 34 GTCAC ^GT[GC]AC
TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 420 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 83 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 498 CCGC CCGC(-3/-1)
681 CCGC
AflIII 77 ACATGT A^C[GA][CT]GT
721 ACGTGT
AluI 291 AGCT AG^CT
704 AGCT
AlwI 315 GATCC GGATC(4/5)
83 GGATC
BcnI 89 CCGGG CC^[GC]GG
171 CCGGG
BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG
479 CCATGG
BsrDI 555 CATTGC GCAATG(2/0)
128 GCAATG
DpnII 84 GATC ^GATC
315 GATC
EaeI 194 TGGCCA [CT]^GGCC[GA]
377 TGGCCG
HapII 89 CCGG C^CGG
171 CCGG
HpaII 89 CCGG C^CGG
171 CCGG
HphI 882 GGTGA GGTGA(8/7)
27 TCACC
MaeIII 34 GTCAC ^GT[ACGT]AC
812 GTTAC
MboI 84 GATC ^GATC
315 GATC
MslI 327 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
435 CATTAGCATG
MspI 89 CCGG C^CGG
171 CCGG
NciI 89 CCGGG CC^[GC]GG
171 CCGGG
NdeII 84 GATC ^GATC
315 GATC
PleI 165 GACTC GAGTC(4/5)
740 GAGTC
Sau3AI 84 GATC ^GATC
315 GATC
SexAI 632 ACCTGGT A^CC[AT]GGT
807 ACCAGGT
TaqI 789 TCGA T^CGA
900 TCGA
TfiI 53 GAATC G^A[AT]TC
897 GATTC
Tsp509I 453 AATT ^AATT
563 AATT
TthHB8I 789 TCGA T^CGA
900 TCGA
XcmI 206 CCACTATTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
318 CCATGCCTTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AspHI BbsI BmyI BpuAI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI EcoRII HaeIII
HinfI MboII MnlI MvaI MwoI
NlaIII NspI Sau96I ScrFI SfaNI