Restriction map for
pFN21AE6070
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KspI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI SwaI TaqI TfiI
Tth111I TthHB8I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 852 GGTACC G^GTACC
AccII 814 CGCG CG^CG
AcsI 899 AAATTC [GA]^AATT[CT]
AlwI 83 GGATC GGATC(4/5)
Aor51HI 497 AGCGCT AGC^GCT
ApoI 899 AAATTC [GA]^AATT[CT]
Asp700I 495 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 852 GGTACC G^GTACC
AvaII 175 GGACC G^G[AT]CC
BanI 852 GGTACC G^G[CT][GA]CC
BbvI 20 GCAGC GCAGC(8/12)
BpmI 550 CTGGAG CTGGAG(16/14)
BsaAI 373 TACGTG [CT]AC^GT[GA]
BsaBI 279 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 70 GGTCTC GGTCTC(1/5)
BseRI 466 CTCCTC GAGGAG(10/8)
BsgI 618 CTGCAC GTGCAG(16/14)
Bsp1407I 819 TGTACA T^GTACA
BspHI 557 TCATGA T^CATGA
BspMI 417 ACCTGC ACCTGC(4/8)
BsrDI 687 CATTGC GCAATG(2/0)
BsrGI 819 TGTACA T^GTACA
BstUI 814 CGCG CG^CG
BstXI 242 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 83 GGATCT [GA]^GATC[CT]
Cfr13I 175 GGACC G^G[ACGT]CC
DrdI 400 GACTCCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 377 TGGCCG [CT]^GGCC[GA]
Eco47III 497 AGCGCT AGC^GCT
FauI 812 CCCGC CCCGC(4/6)
Fnu4HI 20 GCAGC GC^[ACGT]GC
HaeII 497 AGCGCT [GA]GCGC^[CT]
ItaI 20 GCAGC GC^[ACGT]GC
KpnI 852 GGTACC GGTAC^C
MamI 279 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 83 GGATCT [GA]^GATC[CT]
MvnI 814 CGCG CG^CG
MwoI 240 GCCCACTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 201 GCTAGC G^CTAGC
PflMI 519 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 557 TCATGA T^CATGA
Sau96I 175 GGACC G^G[ACGT]CC
SfcI 879 CTACAG C^T[GA][CT]AG
SphI 197 GCATGC GCATG^C
SspBI 819 TGTACA T^GTACA
StuI 219 AGGCCT AGG^CCT
TseI 20 GCAGC G^C[AT]GC
Van91I 519 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 63 TCTAGA T^CTAGA
XhoII 83 GGATCT [GA]^GATC[CT]
XmnI 495 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 369 CCGC CCGC(-3/-1)
813 CCGC
AspHI 461 GTGCTC G[AT]GC[AT]^C
679 GTGCTC
BanII 601 GGGCTC G[GA]GC[CT]^C
713 GAGCCC
BbsI 327 GTCTTC GAAGAC(2/6)
542 GTCTTC
BlpI 441 GCTAAGC GC^T[ACGT]AGC
681 GCTCAGC
Bpu1102I 441 GCTAAGC GC^T[ACGT]AGC
681 GCTCAGC
BpuAI 327 GTCTTC GAAGAC(2/6)
542 GTCTTC
BsiHKAI 461 GTGCTC G[AT]GC[AT]^C
679 GTGCTC
BssKI 549 CCTGG ^CC[ACGT]GG
699 CCAGG
BstNI 549 CCTGG CC^[AT]GG
699 CCAGG
CelII 441 GCTAAGC GC^T[ACGT]AGC
681 GCTCAGC
CfoI 498 GCGC GCG^C
909 GCGC
DpnII 84 GATC ^GATC
555 GATC
EcoRII 549 CCTGG ^CC[AT]GG
699 CCAGG
HhaI 498 GCGC GCG^C
909 GCGC
HinP1I 498 GCGC G^CGC
909 GCGC
MaeII 146 ACGT A^CGT
374 ACGT
MaeIII 208 GTCAC ^GT[ACGT]AC
939 GTCAC
MboI 84 GATC ^GATC
555 GATC
MseI 14 TTAA T^TAA
917 TTAA
MvaI 549 CCTGG CC^[AT]GG
699 CCAGG
NcoI 75 CCATGG C^CATGG
171 CCATGG
NdeII 84 GATC ^GATC
555 GATC
NspI 197 GCATGC [GA]CATG^[CT]
421 GCATGT
SapI 306 GCTCTTC GCTCTTC(1/4)
753 GCTCTTC
Sau3AI 84 GATC ^GATC
555 GATC
ScrFI 549 CCTGG CC^[ACGT]GG
699 CCAGG
Tru9I 14 TTAA T^TAA
917 TTAA
Tsp45I 208 GTCAC ^GT[GC]AC
939 GTCAC
Tsp509I 6 AATT ^AATT
900 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BstF5I
Cac8I DdeI DsaI EarI EcoT14I
FokI HaeIII HinfI HphI Ksp632I
MaeI MboII MnlI MslI NlaIII
NlaIV PleI RsaI SfaNI StyI
TspRI