Restriction map for
pFN21AE6002
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 886 GTCTAC GT^[AC][GT]AC
AciI 378 CCGC CCGC(-3/-1)
AluI 314 AGCT AG^CT
Aor51HI 170 AGCGCT AGC^GCT
AspHI 278 GAGCAC G[AT]GC[AT]^C
AvaII 828 GGTCC G^G[AT]CC
AviII 680 TGCGCA TGC^GCA
BamHI 10 GGATCC G^GATCC
BanI 816 GGCACC G^G[CT][GA]CC
BbsI 897 GAAGAC GAAGAC(2/6)
BglI 700 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 310 GCTCAGC GC^T[ACGT]AGC
BmyI 278 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 310 GCTCAGC GC^T[ACGT]AGC
BpuAI 897 GAAGAC GAAGAC(2/6)
BsaBI 93 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 141 CTGCAC GTGCAG(16/14)
BsiHKAI 278 GAGCAC G[AT]GC[AT]^C
BsmBI 624 CGTCTC CGTCTC(1/5)
Bsp1286I 278 GAGCAC G[AGT]GC[ACT]^C
BspHI 180 TCATGA T^CATGA
BsrI 529 CCAGT ACTGG(1/-1)
BssSI 281 CACGAG CACGAG(-5/-1)
BstXI 798 CCATCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 310 GCTCAGC GC^T[ACGT]AGC
DraII 722 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 170 AGCGCT AGC^GCT
EcoNI 825 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 722 AGGCCCT [GA]G^G[ACGT]CC[CT]
FspI 680 TGCGCA TGC^GCA
HaeII 170 AGCGCT [GA]GCGC^[CT]
HgaI 924 GACGC GACGC(5/10)
HphI 422 TCACC GGTGA(8/7)
MamI 93 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 343 CTCGAG C^TCGAG
PshAI 223 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 180 TCATGA T^CATGA
SapI 762 GCTCTTC GCTCTTC(1/4)
SexAI 824 ACCTGGT A^CC[AT]GGT
SmaI 132 CCCGGG CCC^GGG
SmlI 343 CTCGAG C^T[CT][GA]AG
Tsp45I 922 GTGAC ^GT[GC]AC
XhoI 343 CTCGAG C^TCGAG
XmaCI 132 CCCGGG C^CCGGG
XmaI 132 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaI 132 CCCGGG C^[CT]CG[GA]G
343 CTCGAG
BpmI 702 CTCCAG CTGGAG(16/14)
73 CTGGAG
BsaI 229 GGTCTC GGTCTC(1/5)
453 GGTCTC
BsoBI 132 CCCGGG C^[CT]CG[GA]G
343 CTCGAG
Cfr13I 723 GGCCC G^G[ACGT]CC
828 GGTCC
HapII 133 CCGG C^CGG
912 CCGG
HpaII 133 CCGG C^CGG
912 CCGG
MaeIII 922 GTGAC ^GT[ACGT]AC
938 GTTAC
MseI 161 TTAA T^TAA
728 TTAA
MslI 745 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
771 CACTCCCATG
MspI 133 CCGG C^CGG
912 CCGG
PleI 643 GACTC GAGTC(4/5)
346 GAGTC
Sau96I 723 GGCCC G^G[ACGT]CC
828 GGTCC
SphI 80 GCATGC GCATG^C
927 GCATGC
TaqI 344 TCGA T^CGA
918 TCGA
Tru9I 161 TTAA T^TAA
728 TTAA
TthHB8I 344 TCGA T^CGA
918 TCGA
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AlwI BbvI BcnI BsaJI BsiYI
BslI BsmAI BssKI BstF5I BstNI
BstYI Cac8I CfoI DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI Ksp632I
MboI MboII MflI MnlI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI Sau3AI ScrFI SfaNI SfcI
TfiI TseI Tsp509I TspRI XhoII