Restriction map for
pFN21AE5967
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BclI BcnI BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MaeIII MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AspHI 739 GTGCTC G[AT]GC[AT]^C
AvaII 360 GGACC G^G[AT]CC
BamHI 47 GGATCC G^GATCC
BanI 843 GGTGCC G^G[CT][GA]CC
BbvI 772 GCTGC GCAGC(8/12)
BciVI 546 GGATAC GTATCC(6/5)
BfaI 410 CTAG C^TAG
BglI 190 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 902 AGATCT A^GATCT
BsaBI 42 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 225 CTCCTC GAGGAG(10/8)
BsgI 126 CTGCAC GTGCAG(16/14)
BsiHKAI 739 GTGCTC G[AT]GC[AT]^C
BsmI 455 GCATTC GAATGC(1/-1)
BspHI 165 TCATGA T^CATGA
BspMI 104 ACCTGC ACCTGC(4/8)
BsrI 57 ACTGG ACTGG(1/-1)
BstF5I 84 CATCC GGATG(2/0)
BstXI 551 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 266 CCATGG C^C[GA][CT]GG
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 301 GAATTC G^AATTC
EcoT14I 266 CCATGG C^C[AT][AT]GG
EcoT22I 778 ATGCAT ATGCA^T
FokI 84 CATCC GGATG(9/13)
HindIII 28 AAGCTT A^AGCTT
MaeI 410 CTAG C^TAG
MamI 42 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 266 CCATGG C^CATGG
NsiI 778 ATGCAT ATGCA^T
NspI 63 ACATGC [GA]CATG^[CT]
PleI 882 GACTC GAGTC(4/5)
PshAI 579 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 165 TCATGA T^CATGA
ScaI 339 AGTACT AGT^ACT
SspI 500 AATATT AAT^ATT
StyI 266 CCATGG C^C[AT][AT]GG
TaqI 885 TCGA T^CGA
TseI 772 GCTGC G^C[AT]GC
TthHB8I 885 TCGA T^CGA
XcmI 758 CCATCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
847 CCGC
AcsI 272 GAATTT [GA]^AATT[CT]
301 GAATTC
AluI 29 AGCT AG^CT
557 AGCT
ApoI 272 GAATTT [GA]^AATT[CT]
301 GAATTC
BanII 154 GAGCCC G[GA]GC[CT]^C
169 GAGCCC
BsmAI 202 GTCTC GTCTC(1/5)
721 GTCTC
BsmFI 432 GGGAC GGGAC(10/14)
219 GTCCC
BsrDI 603 CATTGC GCAATG(2/0)
678 CATTGC
BssKI 52 CCAGG ^CC[ACGT]GG
768 CCTGG
BstNI 52 CCAGG CC^[AT]GG
768 CCTGG
BstYI 47 GGATCC [GA]^GATC[CT]
902 AGATCT
EcoRII 52 CCAGG ^CC[AT]GG
768 CCTGG
Fnu4HI 772 GCTGC GC^[ACGT]GC
846 GCCGC
ItaI 772 GCTGC GC^[ACGT]GC
846 GCCGC
MflI 47 GGATCC [GA]^GATC[CT]
902 AGATCT
MslI 321 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
727 CACATCTGTG
MvaI 52 CCAGG CC^[AT]GG
768 CCTGG
NlaIV 47 GGATCC GG[ACGT]^[ACGT]CC
843 GGTGCC
ScrFI 52 CCAGG CC^[ACGT]GG
768 CCTGG
SfaNI 682 GCATC GCATC(5/9)
780 GCATC
TspRI 321 CACTG CA[GC]TG[ACGT][ACGT]^
632 CACTG
XhoII 47 GGATCC [GA]^GATC[CT]
902 AGATCT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AflII AlwI BbsI BfrI
BmyI BpuAI BsaJI BsiYI BslI
Bsp1286I Cac8I Cfr13I DdeI DpnII
HaeIII HinfI HphI MboI MboII
MnlI MseI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I SmlI TfiI
Tru9I Tsp509I