Restriction map for
pFN21AE5529
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
MvnI MwoI NaeI NarI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 755 GTAC GT^AC
AvaI 353 CCCGGG C^[CT]CG[GA]G
AvaII 507 GGTCC G^G[AT]CC
BalI 914 TGGCCA TGG^CCA
BpmI 415 CTGGAG CTGGAG(16/14)
BseRI 124 CTCCTC GAGGAG(10/8)
BsgI 824 GTGCAG GTGCAG(16/14)
BsmFI 681 GGGAC GGGAC(10/14)
BsmI 133 GCATTC GAATGC(1/-1)
BsoBI 353 CCCGGG C^[CT]CG[GA]G
BsrDI 195 CATTGC GCAATG(2/0)
BsrI 175 CCAGT ACTGG(1/-1)
BssSI 601 CACGAG CACGAG(-5/-1)
BstYI 164 GGATCT [GA]^GATC[CT]
DraI 367 TTTAAA TTT^AAA
DrdI 499 GACAGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 914 TGGCCA [CT]^GGCC[GA]
EarI 877 CTCTTC CTCTTC(1/4)
HapII 354 CCGG C^CGG
HindIII 371 AAGCTT A^AGCTT
HpaII 354 CCGG C^CGG
HphI 513 GGTGA GGTGA(8/7)
Ksp632I 877 CTCTTC CTCTTC(1/4)
MaeII 716 ACGT A^CGT
MflI 164 GGATCT [GA]^GATC[CT]
MluNI 914 TGGCCA TGG^CCA
MscI 914 TGGCCA TGG^CCA
MspI 354 CCGG C^CGG
NspI 399 GCATGC [GA]CATG^[CT]
PflMI 656 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 755 GTAC GT^AC
SfcI 903 CTGTAG C^T[GA][CT]AG
SmaI 353 CCCGGG CCC^GGG
SphI 399 GCATGC GCATG^C
TfiI 33 GAATC G^A[AT]TC
Tsp509I 40 AATT ^AATT
Van91I 656 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 194 CCATTGCCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 164 GGATCT [GA]^GATC[CT]
XmaCI 353 CCCGGG C^CCGGG
XmaI 353 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 152 GTGCTC G[AT]GC[AT]^C
898 GTGCTC
BbvI 286 GCAGC GCAGC(8/12)
1002 GCTGC
BclI 638 TGATCA T^GATCA
851 TGATCA
BcnI 353 CCCGG CC^[GC]GG
354 CCGGG
BmyI 152 GTGCTC G[AGT]GC[ACT]^C
898 GTGCTC
BsiHKAI 152 GTGCTC G[AT]GC[AT]^C
898 GTGCTC
BsmAI 246 GAGAC GTCTC(1/5)
185 GTCTC
Bsp1286I 152 GTGCTC G[AGT]GC[ACT]^C
898 GTGCTC
BstF5I 613 CATCC GGATG(2/0)
503 GGATG
Cfr13I 507 GGTCC G^G[ACGT]CC
774 GGCCC
DsaI 80 CCATGG C^C[GA][CT]GG
437 CCATGG
FbaI 638 TGATCA T^GATCA
851 TGATCA
Fnu4HI 286 GCAGC GC^[ACGT]GC
1002 GCTGC
FokI 613 CATCC GGATG(9/13)
503 GGATG
ItaI 286 GCAGC GC^[ACGT]GC
1002 GCTGC
MslI 381 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
817 CACATCTGTG
MspA1I 287 CAGCTG C[AC]G^C[GT]G
1000 CAGCTG
NciI 353 CCCGG CC^[GC]GG
354 CCGGG
NcoI 80 CCATGG C^CATGG
437 CCATGG
PleI 248 GACTC GAGTC(4/5)
418 GAGTC
PvuII 287 CAGCTG CAG^CTG
1000 CAGCTG
Sau96I 507 GGTCC G^G[ACGT]CC
774 GGCCC
SspI 87 AATATT AAT^ATT
919 AATATT
TseI 286 GCAGC G^C[AT]GC
1002 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AlwI BbsI BpuAI
BsaJI BsiYI BslI BssKI BstNI
Cac8I DdeI DpnII EcoRII EcoT14I
HaeIII HinfI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
Sau3AI ScrFI StyI Tru9I Tsp45I
TspRI