Restriction map for
pFN21AE5488
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BpmI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI Fnu4HI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI ItaI
KasI KpnI KspI MaeI MaeII
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TseI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 744 GCGG CCGC(-3/-1)
Asp700I 747 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 898 GTGCTC G[AT]GC[AT]^C
AvaI 308 CCCGAG C^[CT]CG[GA]G
BbvCI 717 CCTCAGC CCTCAGC(-5/-2)
BclI 701 TGATCA T^GATCA
BlpI 61 GCTGAGC GC^T[ACGT]AGC
BmyI 898 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 61 GCTGAGC GC^T[ACGT]AGC
BsaBI 120 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 898 GTGCTC G[AT]GC[AT]^C
BsmBI 156 CGTCTC CGTCTC(1/5)
BsmFI 261 GTCCC GGGAC(10/14)
BsmI 89 GCATTC GAATGC(1/-1)
BsoBI 308 CCCGAG C^[CT]CG[GA]G
Bsp1286I 898 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 268 TGTACA T^GTACA
BspMI 760 ACCTGC ACCTGC(4/8)
BsrGI 268 TGTACA T^GTACA
BssKI 94 CCAGG ^CC[ACGT]GG
BstNI 94 CCAGG CC^[AT]GG
BstXI 825 CCATCGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 119 GGATCT [GA]^GATC[CT]
Cac8I 328 GCCTGC GC[ACGT]^[ACGT]GC
CelII 61 GCTGAGC GC^T[ACGT]AGC
CfoI 646 GCGC GCG^C
DsaI 392 CCATGG C^C[GA][CT]GG
EarI 736 GAAGAG CTCTTC(1/4)
EcoRII 94 CCAGG ^CC[AT]GG
FbaI 701 TGATCA T^GATCA
HhaI 646 GCGC GCG^C
HinP1I 646 GCGC G^CGC
Ksp632I 736 GAAGAG CTCTTC(1/4)
MaeIII 422 GTTAC ^GT[ACGT]AC
MamI 120 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 119 GGATCT [GA]^GATC[CT]
MvaI 94 CCAGG CC^[AT]GG
NcoI 392 CCATGG C^CATGG
NlaIV 200 GGAGCC GG[ACGT]^[ACGT]CC
NspI 482 GCATGT [GA]CATG^[CT]
PleI 373 GAGTC GAGTC(4/5)
PstI 546 CTGCAG CTGCA^G
ScaI 113 AGTACT AGT^ACT
ScrFI 94 CCAGG CC^[ACGT]GG
SspBI 268 TGTACA T^GTACA
StuI 96 AGGCCT AGG^CCT
TaqI 708 TCGA T^CGA
TfiI 936 GATTC G^A[AT]TC
TthHB8I 708 TCGA T^CGA
XhoII 119 GGATCT [GA]^GATC[CT]
XmnI 747 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AluI 256 AGCT AG^CT
992 AGCT
BbsI 18 GTCTTC GAAGAC(2/6)
292 GTCTTC
BpuAI 18 GTCTTC GAAGAC(2/6)
292 GTCTTC
BsgI 606 CTGCAC GTGCAG(16/14)
762 CTGCAC
HinfI 373 GAGTC G^A[ACGT]TC
936 GATTC
MseI 929 TTAA T^TAA
961 TTAA
SfaNI 629 GCATC GCATC(5/9)
680 GCATC
SfcI 546 CTGCAG C^T[GA][CT]AG
659 CTACAG
Tru9I 929 TTAA T^TAA
961 TTAA
Tsp509I 462 AATT ^AATT
963 AATT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AlwI AvaII BalI BsaJI
BsiYI BslI BsmAI BsrDI BsrI
BstF5I Cfr13I DdeI DpnII EaeI
EcoT14I FokI HaeIII MboI MboII
MluNI MnlI MscI MslI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
StyI TspRI