Restriction map for
pFN21AE6085
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HindIII HpaI HpaII KasI KpnI
KspI MaeII MfeI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 402 CCGC CCGC(-3/-1)
AspI 247 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 787 GTCTTC GAAGAC(2/6)
BciVI 83 GTATCC GTATCC(6/5)
BfaI 250 CTAG C^TAG
BglII 929 AGATCT A^GATCT
BpuAI 787 GTCTTC GAAGAC(2/6)
BsaBI 297 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 577 CTGCAC GTGCAG(16/14)
BspMI 757 ACCTGC ACCTGC(4/8)
BsrDI 425 CATTGC GCAATG(2/0)
BsrI 284 CCAGT ACTGG(1/-1)
BstEII 160 GGTAACC G^GT[ACGT]ACC
BstPI 160 GGTAACC G^GT[ACGT]ACC
BstYI 929 AGATCT [GA]^GATC[CT]
CfoI 429 GCGC GCG^C
DdeI 651 CTCAG C^T[ACGT]AG
DraIII 959 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 610 GACACCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 725 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 160 GGTAACC G^GT[ACGT]ACC
HhaI 429 GCGC GCG^C
HinP1I 429 GCGC G^CGC
HincII 895 GTCAAC GT[CT]^[GA]AC
HindII 895 GTCAAC GT[CT]^[GA]AC
HphI 266 TCACC GGTGA(8/7)
MaeI 250 CTAG C^TAG
MamI 297 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 929 AGATCT [GA]^GATC[CT]
MspA1I 374 CAGCTG C[AC]G^C[GT]G
NcoI 389 CCATGG C^CATGG
PflFI 247 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 12 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 941 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 168 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 374 CAGCTG CAG^CTG
SfcI 890 CTGTAG C^T[GA][CT]AG
TaqI 314 TCGA T^CGA
Tsp45I 559 GTCAC ^GT[GC]AC
Tth111I 247 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 314 TCGA T^CGA
Van91I 12 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 929 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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BanII 208 GAGCCC G[GA]GC[CT]^C
808 GGGCTC
BmyI 208 GAGCCC G[AGT]GC[ACT]^C
808 GGGCTC
BseRI 258 CTCCTC GAGGAG(10/8)
847 CTCCTC
BsmFI 22 GTCCC GGGAC(10/14)
836 GTCCC
Bsp1286I 208 GAGCCC G[AGT]GC[ACT]^C
808 GGGCTC
Cac8I 117 GCTGGC GC[ACGT]^[ACGT]GC
384 GCTGGC
DpnII 312 GATC ^GATC
930 GATC
DraII 96 GGGGCCT [GA]G^G[ACGT]CC[CT]
941 GGGTCCT
DsaI 365 CCGTGG C^C[GA][CT]GG
389 CCATGG
EcoO109I 96 GGGGCCT [GA]G^G[ACGT]CC[CT]
941 GGGTCCT
EcoT14I 146 CCTTGG C^C[AT][AT]GG
389 CCATGG
MaeIII 161 GTAAC ^GT[ACGT]AC
559 GTCAC
MboI 312 GATC ^GATC
930 GATC
MwoI 355 GCTCTGTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
739 GCACTCAAGGC
NdeII 312 GATC ^GATC
930 GATC
Sau3AI 312 GATC ^GATC
930 GATC
SfaNI 302 GCATC GCATC(5/9)
761 GCATC
SmlI 526 CTCAAG C^T[CT][GA]AG
742 CTCAAG
StyI 146 CCTTGG C^C[AT][AT]GG
389 CCATGG
TspRI 128 CACTG CA[GC]TG[ACGT][ACGT]^
512 CACTG
XcmI 269 CCATGCCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
614 CCAGGGTCAATGTGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AvaII BalI BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cfr13I EaeI EarI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MboII MluNI MnlI MscI
MslI MvaI NlaIII NlaIV PleI
RsaI Sau96I ScrFI TseI