Restriction map for
pFN21AE5496
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmFI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI Ksp632I KspI MamI
MfeI MflI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 321 GGTACC G^GTACC
AcsI 491 GAATTC [GA]^AATT[CT]
AflIII 808 ACATGT A^C[GA][CT]GT
ApoI 491 GAATTC [GA]^AATT[CT]
Asp718I 321 GGTACC G^GTACC
AspHI 628 GAGCAC G[AT]GC[AT]^C
BanI 321 GGTACC G^G[CT][GA]CC
BciVI 137 GTATCC GTATCC(6/5)
BclI 935 TGATCA T^GATCA
BfaI 919 CTAG C^TAG
BsaAI 931 TACGTG [CT]AC^GT[GA]
BsaI 769 GGTCTC GGTCTC(1/5)
BsiHKAI 628 GAGCAC G[AT]GC[AT]^C
BsmBI 421 GAGACG CGTCTC(1/5)
BsmI 26 GCATTC GAATGC(1/-1)
Bsp1407I 191 TGTACA T^GTACA
BspLU11I 808 ACATGT A^CATGT
BsrDI 729 CATTGC GCAATG(2/0)
BsrGI 191 TGTACA T^GTACA
BstAPI 338 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 936 GATC ^GATC
DraIII 179 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 440 CCATGG C^C[GA][CT]GG
EcoRI 491 GAATTC G^AATTC
EcoT14I 440 CCATGG C^C[AT][AT]GG
EcoT22I 1015 ATGCAT ATGCA^T
FauI 990 GCGGG CCCGC(4/6)
FbaI 935 TGATCA T^GATCA
HincII 5 GTCAAC GT[CT]^[GA]AC
HindII 5 GTCAAC GT[CT]^[GA]AC
KpnI 321 GGTACC GGTAC^C
MaeI 919 CTAG C^TAG
MaeII 932 ACGT A^CGT
MaeIII 226 GTGAC ^GT[ACGT]AC
MboI 936 GATC ^GATC
MspA1I 413 CAGCTG C[AC]G^C[GT]G
NcoI 440 CCATGG C^CATGG
NdeII 936 GATC ^GATC
NheI 918 GCTAGC G^CTAGC
NsiI 1015 ATGCAT ATGCA^T
PflMI 99 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 579 GAGTC GAGTC(4/5)
PvuII 413 CAGCTG CAG^CTG
Sau3AI 936 GATC ^GATC
SfcI 681 CTACAG C^T[GA][CT]AG
SspBI 191 TGTACA T^GTACA
StyI 440 CCATGG C^C[AT][AT]GG
TfiI 244 GATTC G^A[AT]TC
Tsp45I 226 GTGAC ^GT[GC]AC
Van91I 99 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AvaII 85 GGTCC G^G[AT]CC
977 GGACC
BalI 527 TGGCCA TGG^CCA
641 TGGCCA
BanII 259 GAGCCC G[GA]GC[CT]^C
711 GGGCTC
BseRI 312 CTCCTC GAGGAG(10/8)
774 CTCCTC
BstXI 410 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
562 CCACTGAGTTGG
Cfr13I 85 GGTCC G^G[ACGT]CC
977 GGACC
EaeI 527 TGGCCA [CT]^GGCC[GA]
641 TGGCCA
HinfI 244 GATTC G^A[ACGT]TC
579 GAGTC
MluNI 527 TGGCCA TGG^CCA
641 TGGCCA
MscI 527 TGGCCA TGG^CCA
641 TGGCCA
Sau96I 85 GGTCC G^G[ACGT]CC
977 GGACC
SmlI 503 CTCAAG C^T[CT][GA]AG
754 CTCAAG
Tsp509I 492 AATT ^AATT
750 AATT
TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^
563 CACTG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cac8I DdeI
EcoRII Fnu4HI FokI HaeIII HphI
ItaI MboII MnlI MseI MslI
MvaI MwoI NlaIII NlaIV NspI
RsaI ScrFI SfaNI Tru9I TseI