Restriction map for
pFN21AE6005
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 183 GTAC GT^AC
AflIII 724 ACATGT A^C[GA][CT]GT
AluI 583 AGCT AG^CT
AvaI 455 CCCGGG C^[CT]CG[GA]G
BanI 742 GGTGCC G^G[CT][GA]CC
BbvCI 230 CCTCAGC CCTCAGC(-5/-2)
BfaI 581 CTAG C^TAG
BlpI 101 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 101 GCTTAGC GC^T[ACGT]AGC
BsaBI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 228 CTCCTC GAGGAG(10/8)
BsmAI 730 GTCTC GTCTC(1/5)
BsoBI 455 CCCGGG C^[CT]CG[GA]G
BspLU11I 724 ACATGT A^CATGT
BstXI 209 CCATCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 83 GGATCT [GA]^GATC[CT]
CelII 101 GCTTAGC GC^T[ACGT]AGC
HapII 456 CCGG C^CGG
HpaII 456 CCGG C^CGG
MaeI 581 CTAG C^TAG
MaeIII 463 GTGAC ^GT[ACGT]AC
MamI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 83 GGATCT [GA]^GATC[CT]
MseI 22 TTAA T^TAA
MspI 456 CCGG C^CGG
NspI 724 ACATGT [GA]CATG^[CT]
PflMI 254 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 723 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 183 GTAC GT^AC
SapI 849 GCTCTTC GCTCTTC(1/4)
SexAI 215 ACCTGGT A^CC[AT]GGT
SmaI 455 CCCGGG CCC^GGG
StuI 60 AGGCCT AGG^CCT
TaqI 212 TCGA T^CGA
TfiI 54 GATTC G^A[AT]TC
Tru9I 22 TTAA T^TAA
Tsp45I 463 GTGAC ^GT[GC]AC
Tsp509I 838 AATT ^AATT
TthHB8I 212 TCGA T^CGA
Van91I 254 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 83 GGATCT [GA]^GATC[CT]
XmaCI 455 CCCGGG C^CCGGG
XmaI 455 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 83 GGATC GGATC(4/5)
270 GGATC
AspI 214 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACTGGGTC
BcnI 455 CCCGG CC^[GC]GG
456 CCGGG
BglI 196 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
292 GCCTGTCTGGC
BspMI 71 ACCTGC ACCTGC(4/8)
635 ACCTGC
DraII 407 AGGTCCT [GA]G^G[ACGT]CC[CT]
418 GGGTCCC
DsaI 356 CCATGG C^C[GA][CT]GG
860 CCATGG
EarI 777 CTCTTC CTCTTC(1/4)
850 CTCTTC
EcoO109I 407 AGGTCCT [GA]G^G[ACGT]CC[CT]
418 GGGTCCC
FauI 704 CCCGC CCCGC(4/6)
800 CCCGC
Ksp632I 777 CTCTTC CTCTTC(1/4)
850 CTCTTC
NciI 455 CCCGG CC^[GC]GG
456 CCGGG
NcoI 356 CCATGG C^CATGG
860 CCATGG
PflFI 214 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACTGGGTC
PleI 474 GACTC GAGTC(4/5)
337 GAGTC
PpuMI 407 AGGTCCT [GA]G^G[AT]CC[CT]
418 GGGTCCC
Tth111I 214 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACTGGGTC
XcmI 431 CCAAGATTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
614 CCATGTTTATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AvaII BalI BbvI BsaJI
BsgI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MboI MboII MluNI MnlI
MscI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI