Restriction map for
pFN21AE2791
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# NO CUT ENZYMES ( 73 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AvrII
BciVI BclI BfrI BlnI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KpnI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI ScaI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 2614 GACGTC GACGT^C
AcsI 2176 AAATTC [GA]^AATT[CT]
Aor51HI 2987 AGCGCT AGC^GCT
ApoI 2176 AAATTC [GA]^AATT[CT]
Asp700I 2388 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 979 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 263 TGCGCA TGC^GCA
BamHI 305 GGATCC G^GATCC
BbeI 2115 GGCGCC GGCGC^C
BbrPI 2144 CACGTG CAC^GTG
BbvCI 603 GCTGAGG CCTCAGC(-5/-2)
BsaAI 2144 CACGTG [CT]AC^GT[GA]
BsaBI 2547 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1684 GAGACG CGTCTC(1/5)
BsmI 446 GCATTC GAATGC(1/-1)
BspHI 2909 TCATGA T^CATGA
BspLU11I 2969 ACATGT A^CATGT
BsrDI 3261 CATTGC GCAATG(2/0)
BstAPI 351 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 2987 AGCGCT AGC^GCT
FspI 263 TGCGCA TGC^GCA
HincII 82 GTCAAC GT[CT]^[GA]AC
HindII 82 GTCAAC GT[CT]^[GA]AC
KasI 2115 GGCGCC G^GCGCC
KspI 1364 CCGCGG CCGC^GG
MamI 2547 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 845 TTAA T^TAA
NarI 2115 GGCGCC GG^CGCC
PflFI 979 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2144 CACGTG CAC^GTG
PmlI 2144 CACGTG CAC^GTG
RcaI 2909 TCATGA T^CATGA
SacII 1364 CCGCGG CCGC^GG
SfiI 1692 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2115 GGCGCC GGC^GCC
Tru9I 845 TTAA T^TAA
Tsp509I 2177 AATT ^AATT
Tth111I 979 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2388 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2939 ACACGT A^C[GA][CT]GT
2969 ACATGT
BcgI 780 CGATGTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2504 GCACAGTCCTCG
BfaI 796 CTAG C^TAG
1951 CTAG
BglII 1100 AGATCT A^GATCT
2747 AGATCT
BlpI 3048 GCTCAGC GC^T[ACGT]AGC
3094 GCTGAGC
Bpu1102I 3048 GCTCAGC GC^T[ACGT]AGC
3094 GCTGAGC
BsaI 3122 GAGACC GGTCTC(1/5)
1395 GGTCTC
BsiEI 1452 CGGCCG CG[GA][CT]^CG
2697 CGGCCG
CelII 3048 GCTCAGC GC^T[ACGT]AGC
3094 GCTGAGC
DraIII 2380 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3247 CACAGAGTG
DrdI 1156 GACGCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3142 GACCAGAAGGTC
EagI 1452 CGGCCG C^GGCCG
2697 CGGCCG
EclXI 1452 CGGCCG C^GGCCG
2697 CGGCCG
Eco52I 1452 CGGCCG C^GGCCG
2697 CGGCCG
FseI 1329 GGCCGGCC GGCCGG^CC
1453 GGCCGGCC
HinfI 2824 GACTC G^A[ACGT]TC
3316 GAGTC
MaeI 796 CTAG C^TAG
1951 CTAG
NcoI 1616 CCATGG C^CATGG
2516 CCATGG
NspI 342 GCATGT [GA]CATG^[CT]
2969 ACATGT
PleI 2824 GACTC GAGTC(4/5)
3316 GAGTC
PvuII 2572 CAGCTG CAG^CTG
3418 CAGCTG
SexAI 197 ACCTGGT A^CC[AT]GGT
2381 ACCTGGT
SmlI 1054 CTCAAG C^T[CT][GA]AG
1408 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
Sse8387I StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI