Restriction map for
pFN21AE5178
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI MaeI MaeIII
MamI MfeI MluI MluNI MscI
MseI MunI MvaI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I TspRI
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 125 TCCGGA T^CCGGA
AcyI 436 GGCGTC G[GA]^CG[CT]C
AocI 156 CCTCAGG CC^T[ACGT]AGG
Aor51HI 361 AGCGCT AGC^GCT
AvaII 153 GGACC G^G[AT]CC
BamHI 128 GGATCC G^GATCC
BanII 348 GAGCCC G[GA]GC[CT]^C
BcnI 91 CCCGG CC^[GC]GG
BglI 350 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 324 AGATCT A^GATCT
BsaAI 286 TACGTG [CT]AC^GT[GA]
BsaHI 436 GGCGTC G[GA]^CG[CT]C
BsaWI 125 TCCGGA [AT]^CCGG[AT]
BseAI 125 TCCGGA T^CCGGA
BsmFI 439 GTCCC GGGAC(10/14)
BspEI 125 TCCGGA T^CCGGA
BsrFI 29 ACCGGC [GA]^CCGG[CT]
BssKI 91 CCCGG ^CC[ACGT]GG
Bsu36I 156 CCTCAGG CC^T[ACGT]AGG
Cfr10I 29 ACCGGC [GA]^CCGG[CT]
DdeI 157 CTCAG C^T[ACGT]AG
DrdI 271 GACTACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 236 GAAGAG CTCTTC(1/4)
Eco47III 361 AGCGCT AGC^GCT
Eco81I 156 CCTCAGG CC^T[ACGT]AGG
HaeII 361 AGCGCT [GA]GCGC^[CT]
HgaI 437 GCGTC GACGC(5/10)
Hin1I 436 GGCGTC G[GA]^CG[CT]C
Ksp632I 236 GAAGAG CTCTTC(1/4)
KspI 424 CCGCGG CCGC^GG
MroI 125 TCCGGA T^CCGGA
MslI 135 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 91 CCCGG CC^[GC]GG
NlaIV 128 GGATCC GG[ACGT]^[ACGT]CC
NspI 383 GCATGC [GA]CATG^[CT]
SacII 424 CCGCGG CCGC^GG
ScrFI 91 CCCGG CC^[ACGT]GG
SfaNI 176 GCATC GCATC(5/9)
SfcI 378 CTACAG C^T[GA][CT]AG
SphI 383 GCATGC GCATG^C
Tsp509I 163 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 5 CGCG CG^CG
425 CGCG
AfaI 18 GTAC GT^AC
27 GTAC
BmyI 187 GTGCCC G[AGT]GC[ACT]^C
348 GAGCCC
BseRI 391 GAGGAG GAGGAG(10/8)
394 GAGGAG
Bsp1286I 187 GTGCCC G[AGT]GC[ACT]^C
348 GAGCCC
BstUI 5 CGCG CG^CG
425 CGCG
BstYI 128 GGATCC [GA]^GATC[CT]
324 AGATCT
Cac8I 294 GCTGGC GC[ACGT]^[ACGT]GC
383 GCATGC
CfoI 4 GCGC GCG^C
362 GCGC
EcoT14I 134 CCATGG C^C[AT][AT]GG
300 CCATGG
FauI 423 CCCGC CCCGC(4/6)
429 GCGGG
HhaI 4 GCGC GCG^C
362 GCGC
HinP1I 4 GCGC G^CGC
362 GCGC
MboII 236 GAAGA GAAGA(8/7)
71 TCTTC
MflI 128 GGATCC [GA]^GATC[CT]
324 AGATCT
MvnI 5 CGCG CG^CG
425 CGCG
NcoI 134 CCATGG C^CATGG
300 CCATGG
PaeR7I 169 CTCGAG C^TCGAG
388 CTCGAG
RsaI 18 GTAC GT^AC
27 GTAC
SmlI 169 CTCGAG C^T[CT][GA]AG
388 CTCGAG
StyI 134 CCATGG C^C[AT][AT]GG
300 CCATGG
TaqI 170 TCGA T^CGA
389 TCGA
TthHB8I 170 TCGA T^CGA
389 TCGA
XhoI 169 CTCGAG C^TCGAG
388 CTCGAG
XhoII 128 GGATCC [GA]^GATC[CT]
324 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AlwI AvaI BbvI
BsaJI BsgI BsiYI BslI BsoBI
Cfr13I DpnII DsaI Fnu4HI HaeIII
HapII HpaII ItaI MaeII MboI
MnlI MspA1I MspI MwoI NdeII
NlaIII PvuII Sau3AI Sau96I TseI