Restriction map for
pFN21AE2759
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbvCI BciVI BclI BfaI
BfrI BlnI BsiWI BsmI Bsp1407I
BspDI BsrGI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KspI MaeI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp509I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 734 GGTACC G^GTACC
AccIII 20 TCCGGA T^CCGGA
Alw44I 1153 GTGCAC G^TGCAC
AocI 289 CCTGAGG CC^T[ACGT]AGG
ApaLI 1153 GTGCAC G^TGCAC
Asp718I 734 GGTACC G^GTACC
BbrPI 2131 CACGTG CAC^GTG
BcgI 1466 GCAACTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1461 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1461 GCTCAGC GC^T[ACGT]AGC
BsaWI 20 TCCGGA [AT]^CCGG[AT]
BseAI 20 TCCGGA T^CCGGA
BsmBI 1301 CGTCTC CGTCTC(1/5)
BspEI 20 TCCGGA T^CCGGA
BspLU11I 506 ACATGT A^CATGT
BsrFI 606 GCCGGC [GA]^CCGG[CT]
BssHII 864 GCGCGC G^CGCGC
Bsu36I 289 CCTGAGG CC^T[ACGT]AGG
CelII 1461 GCTCAGC GC^T[ACGT]AGC
Cfr10I 606 GCCGGC [GA]^CCGG[CT]
DraIII 1156 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 247 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 289 CCTGAGG CC^T[ACGT]AGG
EcoNI 456 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 1207 AAGCTT A^AGCTT
KpnI 734 GGTACC GGTAC^C
MroI 20 TCCGGA T^CCGGA
NaeI 606 GCCGGC GCC^GGC
NgoMIV 606 GCCGGC G^CCGGC
PflMI 1044 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2131 CACGTG CAC^GTG
PmlI 2131 CACGTG CAC^GTG
SalI 1930 GTCGAC G^TCGAC
SexAI 1481 ACCTGGT A^CC[AT]GGT
SmlI 1939 CTCAAG C^T[CT][GA]AG
StuI 86 AGGCCT AGG^CCT
Van91I 1044 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 506 ACATGT A^C[GA][CT]GT
896 ACGTGT
AspI 103 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
430 GACGGGGTC
BalI 1433 TGGCCA TGG^CCA
2096 TGGCCA
BamHI 23 GGATCC G^GATCC
236 GGATCC
BbeI 1680 GGCGCC GGCGC^C
1754 GGCGCC
BglII 680 AGATCT A^GATCT
848 AGATCT
BsaAI 895 TACGTG [CT]AC^GT[GA]
2131 CACGTG
BsrBI 1509 CCGCTC CCGCTC(-3/-3)
639 GAGCGG
BsrDI 1830 CATTGC GCAATG(2/0)
1848 CATTGC
EcoT14I 1775 CCAAGG C^C[AT][AT]GG
1979 CCAAGG
HaeII 1680 GGCGCC [GA]GCGC^[CT]
1754 GGCGCC
KasI 1680 GGCGCC G^GCGCC
1754 GGCGCC
MluNI 1433 TGGCCA TGG^CCA
2096 TGGCCA
MscI 1433 TGGCCA TGG^CCA
2096 TGGCCA
MseI 200 TTAA T^TAA
938 TTAA
NarI 1680 GGCGCC GG^CGCC
1754 GGCGCC
NspI 506 ACATGT [GA]CATG^[CT]
596 ACATGC
PflFI 103 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
430 GACGGGGTC
PstI 1279 CTGCAG CTGCA^G
1795 CTGCAG
SacI 175 GAGCTC GAGCT^C
1555 GAGCTC
SapI 1080 GCTCTTC GCTCTTC(1/4)
1695 GCTCTTC
SfoI 1680 GGCGCC GGC^GCC
1754 GGCGCC
SmaI 1348 CCCGGG CCC^GGG
1544 CCCGGG
StyI 1775 CCAAGG C^C[AT][AT]GG
1979 CCAAGG
Tru9I 200 TTAA T^TAA
938 TTAA
Tth111I 103 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
430 GACGGGGTC
XmaCI 1348 CCCGGG C^CCGGG
1544 CCCGGG
XmaI 1348 CCCGGG C^CCGGG
1544 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoO65I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MamI
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII