Restriction map for
pFN21AB8927
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I ClaI CpoI DpnII DraIII
DrdI Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MaeI
MaeII MaeIII MamI MboI MfeI
MflI MroI MslI MunI NcoI
NdeI NdeII NheI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 268 GGTACC G^GTACC
AflIII 656 ACGCGT A^C[GA][CT]GT
AlwNI 667 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 217 CCTAAGG CC^T[ACGT]AGG
Aor51HI 512 AGCGCT AGC^GCT
Asp718I 268 GGTACC G^GTACC
AvaII 395 GGTCC G^G[AT]CC
BalI 118 TGGCCA TGG^CCA
BbeI 183 GGCGCC GGCGC^C
BbsI 450 GAAGAC GAAGAC(2/6)
BpuAI 450 GAAGAC GAAGAC(2/6)
BsaI 568 GAGACC GGTCTC(1/5)
BsmBI 48 CGTCTC CGTCTC(1/5)
BsrI 66 CCAGT ACTGG(1/-1)
BssHII 534 GCGCGC G^CGCGC
Bsu36I 217 CCTAAGG CC^T[ACGT]AGG
DraI 346 TTTAAA TTT^AAA
EarI 352 GAAGAG CTCTTC(1/4)
Eco47III 512 AGCGCT AGC^GCT
Eco81I 217 CCTAAGG CC^T[ACGT]AGG
EcoT14I 275 CCAAGG C^C[AT][AT]GG
KasI 183 GGCGCC G^GCGCC
KpnI 268 GGTACC GGTAC^C
Ksp632I 352 GAAGAG CTCTTC(1/4)
MluI 656 ACGCGT A^CGCGT
MluNI 118 TGGCCA TGG^CCA
MscI 118 TGGCCA TGG^CCA
MseI 347 TTAA T^TAA
NaeI 433 GCCGGC GCC^GGC
NarI 183 GGCGCC GG^CGCC
NgoMIV 433 GCCGGC G^CCGGC
NotI 402 GCGGCCGC GC^GGCCGC
NspI 680 GCATGC [GA]CATG^[CT]
PaeR7I 490 CTCGAG C^TCGAG
PstI 556 CTGCAG CTGCA^G
SfaNI 16 GATGC GCATC(5/9)
SfcI 556 CTGCAG C^T[GA][CT]AG
SfiI 432 GGCCGGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 183 GGCGCC GGC^GCC
SmlI 490 CTCGAG C^T[CT][GA]AG
SphI 680 GCATGC GCATG^C
StyI 275 CCAAGG C^C[AT][AT]GG
Tru9I 347 TTAA T^TAA
XhoI 490 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 46 GGCGTC G[GA]^CG[CT]C
183 GGCGCC
AfaI 269 GTAC GT^AC
483 GTAC
BlpI 421 GCTGAGC GC^T[ACGT]AGC
628 GCTGAGC
Bpu1102I 421 GCTGAGC GC^T[ACGT]AGC
628 GCTGAGC
BsaHI 46 GGCGTC G[GA]^CG[CT]C
183 GGCGCC
BsiEI 38 CGGCCG CG[GA][CT]^CG
403 CGGCCG
BsmAI 568 GAGAC GTCTC(1/5)
49 GTCTC
BsmFI 410 GGGAC GGGAC(10/14)
698 GGGAC
CelII 421 GCTGAGC GC^T[ACGT]AGC
628 GCTGAGC
EagI 38 CGGCCG C^GGCCG
403 CGGCCG
EclXI 38 CGGCCG C^GGCCG
403 CGGCCG
Eco52I 38 CGGCCG C^GGCCG
403 CGGCCG
Hin1I 46 GGCGTC G[GA]^CG[CT]C
183 GGCGCC
HinfI 493 GAGTC G^A[ACGT]TC
669 GACTC
HphI 675 GGTGA GGTGA(8/7)
59 TCACC
PleI 669 GACTC GAGTC(4/5)
493 GAGTC
RsaI 269 GTAC GT^AC
483 GTAC
SmaI 297 CCCGGG CCC^GGG
762 CCCGGG
XmaCI 297 CCCGGG C^CCGGG
762 CCCGGG
XmaI 297 CCCGGG C^CCGGG
762 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsoBI Bsp1286I BsrBI BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
KspI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV SacII Sau96I ScrFI TaqI
TseI TthHB8I