Restriction map for
pFN21AE4053
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BalI
BamHI BbrPI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MluNI
MscI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 22 TCCGGA T^CCGGA
AflIII 90 ACACGT A^C[GA][CT]GT
AspI 703 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 37 GAGCCC G[GA]GC[CT]^C
BbeI 282 GGCGCC GGCGC^C
BbsI 73 GAAGAC GAAGAC(2/6)
BcgI 375 GCAACTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 229 GCCGCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 37 GAGCCC G[AGT]GC[ACT]^C
BpmI 502 CTGGAG CTGGAG(16/14)
BpuAI 73 GAAGAC GAAGAC(2/6)
BsaI 580 GGTCTC GGTCTC(1/5)
BseAI 22 TCCGGA T^CCGGA
BseRI 505 GAGGAG GAGGAG(10/8)
BsiEI 154 CGGTCG CG[GA][CT]^CG
BsmAI 581 GTCTC GTCTC(1/5)
Bsp1286I 37 GAGCCC G[AGT]GC[ACT]^C
BspEI 22 TCCGGA T^CCGGA
BstF5I 252 GGATG GGATG(2/0)
DraIII 766 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
FokI 252 GGATG GGATG(9/13)
KasI 282 GGCGCC G^GCGCC
MboII 73 GAAGA GAAGA(8/7)
MroI 22 TCCGGA T^CCGGA
MslI 816 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 282 GGCGCC GG^CGCC
NlaIII 769 CATG CATG^
PflFI 703 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 891 CCTGCAGG CCTGCA^GG
ScaI 725 AGTACT AGT^ACT
SexAI 314 ACCTGGT A^CC[AT]GGT
SfiI 228 GGCCGCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 282 GGCGCC GGC^GCC
SmlI 535 CTCAAG C^T[CT][GA]AG
Sse8387I 891 CCTGCAGG CCTGCA^GG
TfiI 336 GAATC G^A[AT]TC
Tth111I 703 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 548 CCATCGTGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 282 GGCGCC G[GA]^CG[CT]C
622 GACGCC
AfaI 555 GTAC GT^AC
726 GTAC
AlwI 219 GATCC GGATC(4/5)
513 GGATC
BanI 282 GGCGCC G^G[CT][GA]CC
318 GGTGCC
BsaHI 282 GGCGCC G[GA]^CG[CT]C
622 GACGCC
BsaWI 22 TCCGGA [AT]^CCGG[AT]
152 TCCGGT
BsgI 835 CTGCAC GTGCAG(16/14)
163 GTGCAG
BsmFI 620 GGGAC GGGAC(10/14)
106 GTCCC
BsrFI 321 GCCGGT [GA]^CCGG[CT]
679 ACCGGC
BsrI 279 ACTGG ACTGG(1/-1)
452 ACTGG
BstYI 218 AGATCC [GA]^GATC[CT]
513 GGATCT
Cfr10I 321 GCCGGT [GA]^CCGG[CT]
679 ACCGGC
DdeI 379 CTGAG C^T[ACGT]AG
787 CTGAG
DsaI 223 CCGCGG C^C[GA][CT]GG
626 CCGCGG
Hin1I 282 GGCGCC G[GA]^CG[CT]C
622 GACGCC
HincII 604 GTCAAC GT[CT]^[GA]AC
886 GTCAAC
HindII 604 GTCAAC GT[CT]^[GA]AC
886 GTCAAC
KspI 223 CCGCGG CCGC^GG
626 CCGCGG
MaeIII 290 GTCAC ^GT[ACGT]AC
449 GTGAC
MflI 218 AGATCC [GA]^GATC[CT]
513 GGATCT
PflMI 765 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
840 CCAGGCCCTGG
PleI 381 GAGTC GAGTC(4/5)
778 GAGTC
PpuMI 104 GGGTCCC [GA]G^G[AT]CC[CT]
311 AGGACCT
PvuII 118 CAGCTG CAG^CTG
343 CAGCTG
RsaI 555 GTAC GT^AC
726 GTAC
SacII 223 CCGCGG CCGC^GG
626 CCGCGG
SfaNI 196 GATGC GCATC(5/9)
253 GATGC
TaqI 194 TCGA T^CGA
818 TCGA
Tsp45I 290 GTCAC ^GT[GC]AC
449 GTGAC
TthHB8I 194 TCGA T^CGA
818 TCGA
Van91I 765 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
840 CCAGGCCCTGG
XhoII 218 AGATCC [GA]^GATC[CT]
513 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BbvI BcnI BsaJI BsiYI BslI
BspMI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TseI TspRI