Restriction map for
pFN21AE4078
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# NO CUT ENZYMES ( 112 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsiHKAI
BsiWI BspDI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I MamI MfeI
MflI MluI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SapI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1131 GACGTC GACGT^C
AccI 1134 GTCGAC GT^[AC][GT]AC
AccIII 974 TCCGGA T^CCGGA
AlwNI 875 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 689 CCTCAGG CC^T[ACGT]AGG
ApaI 137 GGGCCC GGGCC^C
AspI 733 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 730 GAAGAC GAAGAC(2/6)
BbvCI 1143 CCTCAGC CCTCAGC(-5/-2)
BfaI 376 CTAG C^TAG
BglI 58 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 730 GAAGAC GAAGAC(2/6)
BsaWI 974 TCCGGA [AT]^CCGG[AT]
BseAI 974 TCCGGA T^CCGGA
BsmBI 1168 CGTCTC CGTCTC(1/5)
BsmI 557 GCATTC GAATGC(1/-1)
Bsp1407I 392 TGTACA T^GTACA
BspEI 974 TCCGGA T^CCGGA
BspHI 600 TCATGA T^CATGA
BspMI 1046 ACCTGC ACCTGC(4/8)
BsrDI 747 CATTGC GCAATG(2/0)
BsrGI 392 TGTACA T^GTACA
Bsu36I 689 CCTCAGG CC^T[ACGT]AGG
DpnII 193 GATC ^GATC
DraIII 502 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 68 CCGCGG C^C[GA][CT]GG
Eco81I 689 CCTCAGG CC^T[ACGT]AGG
EcoT14I 671 CCAAGG C^C[AT][AT]GG
HincII 1134 GTCGAC GT[CT]^[GA]AC
HindII 1134 GTCGAC GT[CT]^[GA]AC
KspI 68 CCGCGG CCGC^GG
MaeI 376 CTAG C^TAG
MboI 193 GATC ^GATC
MroI 974 TCCGGA T^CCGGA
NaeI 695 GCCGGC GCC^GGC
NdeII 193 GATC ^GATC
NgoMIV 695 GCCGGC G^CCGGC
NspI 131 ACATGC [GA]CATG^[CT]
PflFI 733 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1027 CTGCAG CTGCA^G
RcaI 600 TCATGA T^CATGA
SacII 68 CCGCGG CCGC^GG
SalI 1134 GTCGAC G^TCGAC
Sau3AI 193 GATC ^GATC
ScaI 590 AGTACT AGT^ACT
SfcI 1027 CTGCAG C^T[GA][CT]AG
SmaI 215 CCCGGG CCC^GGG
SspBI 392 TGTACA T^GTACA
StyI 671 CCAAGG C^C[AT][AT]GG
TaqI 1135 TCGA T^CGA
Tsp509I 409 AATT ^AATT
Tth111I 733 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1135 TCGA T^CGA
XmaCI 215 CCCGGG C^CCGGG
XmaI 215 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 69 CGCG CG^CG
242 CGCG
AvaI 44 CTCGGG C^[CT]CG[GA]G
215 CCCGGG
BalI 512 TGGCCA TGG^CCA
1052 TGGCCA
BanII 137 GGGCCC G[GA]GC[CT]^C
1000 GAGCCC
BbeI 86 GGCGCC GGCGC^C
203 GGCGCC
BpmI 654 CTCCAG CTGGAG(16/14)
306 CTGGAG
BsgI 1114 CTGCAC GTGCAG(16/14)
533 GTGCAG
BsoBI 44 CTCGGG C^[CT]CG[GA]G
215 CCCGGG
BsrFI 695 GCCGGC [GA]^CCGG[CT]
1094 ACCGGC
BstUI 69 CGCG CG^CG
242 CGCG
Cfr10I 695 GCCGGC [GA]^CCGG[CT]
1094 ACCGGC
EcoNI 90 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
150 CCTGGCCCAGG
KasI 86 GGCGCC G^GCGCC
203 GGCGCC
MaeIII 778 GTGAC ^GT[ACGT]AC
863 GTGAC
MboII 549 GAAGA GAAGA(8/7)
730 GAAGA
MluNI 512 TGGCCA TGG^CCA
1052 TGGCCA
MscI 512 TGGCCA TGG^CCA
1052 TGGCCA
MslI 639 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1183 CATCTCTATG
MvnI 69 CGCG CG^CG
242 CGCG
NarI 86 GGCGCC GG^CGCC
203 GGCGCC
PpuMI 824 GGGACCT [GA]G^G[AT]CC[CT]
956 GGGACCT
PshAI 264 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1093 GACCGGCGTC
PvuII 186 CAGCTG CAG^CTG
841 CAGCTG
SfoI 86 GGCGCC GGC^GCC
203 GGCGCC
Tsp45I 778 GTGAC ^GT[GC]AC
863 GTGAC
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BanI BbvI BcnI BmyI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PflMI PleI RsaI Sau96I
ScrFI SfaNI TfiI TseI TspRI
Van91I