Restriction map for
pFN21AE1841
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AciI 898 GCGG CCGC(-3/-1)
AclI 776 AACGTT AA^CGTT
AflIII 586 ACATGT A^C[GA][CT]GT
ApaI 191 GGGCCC GGGCC^C
AseI 622 ATTAAT AT^TAAT
AsnI 622 ATTAAT AT^TAAT
Asp700I 775 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 480 GTGCTC G[AT]GC[AT]^C
BalI 318 TGGCCA TGG^CCA
BsaI 259 GAGACC GGTCTC(1/5)
BsaWI 176 TCCGGT [AT]^CCGG[AT]
BsiHKAI 480 GTGCTC G[AT]GC[AT]^C
BsmAI 259 GAGAC GTCTC(1/5)
BsmFI 233 GGGAC GGGAC(10/14)
BsmI 278 GCATTC GAATGC(1/-1)
BspLU11I 586 ACATGT A^CATGT
BspMI 878 GCAGGT ACCTGC(4/8)
BstBI 380 TTCGAA TT^CGAA
BstYI 575 AGATCC [GA]^GATC[CT]
DraI 951 TTTAAA TTT^AAA
HgaI 12 GCGTC GACGC(5/10)
HincII 499 GTTGAC GT[CT]^[GA]AC
HindII 499 GTTGAC GT[CT]^[GA]AC
HindIII 529 AAGCTT A^AGCTT
HphI 1019 TCACC GGTGA(8/7)
MaeII 777 ACGT A^CGT
MflI 575 AGATCC [GA]^GATC[CT]
MluNI 318 TGGCCA TGG^CCA
MscI 318 TGGCCA TGG^CCA
MslI 325 CATCGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 896 CAGCGG C[AC]G^C[GT]G
NdeI 724 CATATG CA^TATG
NspV 380 TTCGAA TT^CGAA
PshBI 622 ATTAAT AT^TAAT
Psp1406I 776 AACGTT AA^CGTT
ScaI 468 AGTACT AGT^ACT
SfuI 380 TTCGAA TT^CGAA
SphI 828 GCATGC GCATG^C
SspI 946 AATATT AAT^ATT
XhoII 575 AGATCC [GA]^GATC[CT]
XmnI 775 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 180 GTCTAC GT^[AC][GT]AC
1024 GTAGAC
AcsI 616 GAATTC [GA]^AATT[CT]
993 GAATTC
AfaI 469 GTAC GT^AC
882 GTAC
ApoI 616 GAATTC [GA]^AATT[CT]
993 GAATTC
BanII 133 GAGCCC G[GA]GC[CT]^C
191 GGGCCC
BbvI 390 GCAGC GCAGC(8/12)
506 GCAGC
BfaI 239 CTAG C^TAG
649 CTAG
BsaJI 116 CCATGG C^C[ACGT][ACGT]GG
485 CCATGG
BstF5I 548 GGATG GGATG(2/0)
702 GGATG
Cfr13I 191 GGGCC G^G[ACGT]CC
192 GGCCC
DdeI 451 CTGAG C^T[ACGT]AG
1010 CTCAG
DraII 190 AGGGCCC [GA]G^G[ACGT]CC[CT]
191 GGGCCCC
DsaI 116 CCATGG C^C[GA][CT]GG
485 CCATGG
EarI 94 GAAGAG CTCTTC(1/4)
571 GAAGAG
EcoO109I 190 AGGGCCC [GA]G^G[ACGT]CC[CT]
191 GGGCCCC
EcoRI 616 GAATTC G^AATTC
993 GAATTC
EcoT14I 116 CCATGG C^C[AT][AT]GG
485 CCATGG
Fnu4HI 390 GCAGC GC^[ACGT]GC
506 GCAGC
FokI 548 GGATG GGATG(9/13)
702 GGATG
HapII 39 CCGG C^CGG
177 CCGG
HpaII 39 CCGG C^CGG
177 CCGG
ItaI 390 GCAGC GC^[ACGT]GC
506 GCAGC
Ksp632I 94 GAAGAG CTCTTC(1/4)
571 GAAGAG
MaeI 239 CTAG C^TAG
649 CTAG
MseI 623 TTAA T^TAA
952 TTAA
MspI 39 CCGG C^CGG
177 CCGG
MwoI 12 GCGTCCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
204 GCTCTATAAGC
NcoI 116 CCATGG C^CATGG
485 CCATGG
NspI 586 ACATGT [GA]CATG^[CT]
828 GCATGC
RsaI 469 GTAC GT^AC
882 GTAC
Sau96I 191 GGGCC G^G[ACGT]CC
192 GGCCC
SfcI 598 CTACAG C^T[GA][CT]AG
833 CTACAG
StyI 116 CCATGG C^C[AT][AT]GG
485 CCATGG
Tru9I 623 TTAA T^TAA
952 TTAA
TseI 390 GCAGC G^C[AT]GC
506 GCAGC
TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^
604 CAGTG
XcmI 321 CCAGCATCGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
723 CCATATGAAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AlwI BmyI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cac8I
DpnII EaeI EcoRII HaeIII HinfI
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV Sau3AI ScrFI SfaNI
TaqI TfiI Tsp509I TthHB8I