Restriction map for
pFN21AE5930
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 25 GAATTC [GA]^AATT[CT]
AfaI 171 GTAC GT^AC
AflIII 479 ACATGT A^C[GA][CT]GT
AocI 770 CCTTAGG CC^T[ACGT]AGG
Aor51HI 105 AGCGCT AGC^GCT
ApoI 25 GAATTC [GA]^AATT[CT]
Asp700I 674 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 775 GGACC G^G[AT]CC
BcnI 77 CCGGG CC^[GC]GG
BsgI 70 CTGCAC GTGCAG(16/14)
BsiYI 30 CCTGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 30 CCTGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 900 GAGAC GTCTC(1/5)
BspHI 632 TCATGA T^CATGA
BspLU11I 479 ACATGT A^CATGT
BsrDI 129 CATTGC GCAATG(2/0)
BstAPI 63 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 770 CCTTAGG CC^T[ACGT]AGG
CfoI 106 GCGC GCG^C
Eco47III 105 AGCGCT AGC^GCT
Eco81I 770 CCTTAGG CC^T[ACGT]AGG
EcoRI 25 GAATTC G^AATTC
HaeII 105 AGCGCT [GA]GCGC^[CT]
HapII 77 CCGG C^CGG
HhaI 106 GCGC GCG^C
HinP1I 106 GCGC G^CGC
HpaII 77 CCGG C^CGG
HphI 431 TCACC GGTGA(8/7)
MaeII 494 ACGT A^CGT
MfeI 779 CAATTG C^AATTG
MseI 884 TTAA T^TAA
MspI 77 CCGG C^CGG
MunI 779 CAATTG C^AATTG
NciI 77 CCGGG CC^[GC]GG
NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC
NspI 479 ACATGT [GA]CATG^[CT]
PflFI 686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 61 CTGCAG CTGCA^G
RcaI 632 TCATGA T^CATGA
RsaI 171 GTAC GT^AC
SacI 600 GAGCTC GAGCT^C
SfcI 61 CTGCAG C^T[GA][CT]AG
SmlI 290 CTCAAG C^T[CT][GA]AG
TaqI 693 TCGA T^CGA
TfiI 674 GAATC G^A[AT]TC
Tru9I 884 TTAA T^TAA
Tth111I 686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 693 TCGA T^CGA
XmnI 674 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 357 CCGC CCGC(-3/-1)
133 GCGG
AlwNI 64 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
218 CAGTCACTG
BanII 600 GAGCTC G[GA]GC[CT]^C
757 GGGCTC
BlpI 322 GCTGAGC GC^T[ACGT]AGC
441 GCTAAGC
Bpu1102I 322 GCTGAGC GC^T[ACGT]AGC
441 GCTAAGC
BsaJI 551 CCCTGG C^C[ACGT][ACGT]GG
590 CCCTGG
BsrI 472 ACTGG ACTGG(1/-1)
519 CCAGT
BstYI 201 GGATCT [GA]^GATC[CT]
502 AGATCC
Cac8I 72 GCACGC GC[ACGT]^[ACGT]GC
327 GCTGGC
CelII 322 GCTGAGC GC^T[ACGT]AGC
441 GCTAAGC
MflI 201 GGATCT [GA]^GATC[CT]
502 AGATCC
MslI 12 CACAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
162 CACACCCATG
Tsp509I 26 AATT ^AATT
780 AATT
TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^
471 CACTG
XhoII 201 GGATCT [GA]^GATC[CT]
502 AGATCC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI AspHI BbvI BmyI
BsiHKAI Bsp1286I BspMI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIII PleI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I