Restriction map for
pFN21AE6042
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BcnI BfrI BglI BpmI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CfoI Cfr10I
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 247 GTCGAC GT^[AC][GT]AC
AcsI 659 GAATTC [GA]^AATT[CT]
AlwNI 323 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 896 GGGCCC GGGCC^C
ApoI 659 GAATTC [GA]^AATT[CT]
AvrII 342 CCTAGG C^CTAGG
BalI 713 TGGCCA TGG^CCA
BanI 540 GGTGCC G^G[CT][GA]CC
BcgI 537 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 343 CTAG C^TAG
BglII 416 AGATCT A^GATCT
BlnI 342 CCTAGG C^CTAGG
BlpI 186 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC
BsmAI 586 GAGAC GTCTC(1/5)
BsmFI 206 GGGAC GGGAC(10/14)
Bsp1407I 767 TGTACA T^GTACA
BspDI 460 ATCGAT AT^CGAT
BspHI 404 TCATGA T^CATGA
BspMI 718 ACCTGC ACCTGC(4/8)
BsrGI 767 TGTACA T^GTACA
BstAPI 413 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 939 GGTAACC G^GT[ACGT]ACC
BstPI 939 GGTAACC G^GT[ACGT]ACC
BstYI 416 AGATCT [GA]^GATC[CT]
CelII 186 GCTCAGC GC^T[ACGT]AGC
ClaI 460 ATCGAT AT^CGAT
DraIII 730 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 713 TGGCCA [CT]^GGCC[GA]
EarI 748 CTCTTC CTCTTC(1/4)
EcoNI 900 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 939 GGTAACC G^GT[ACGT]ACC
EcoRI 659 GAATTC G^AATTC
EcoT14I 342 CCTAGG C^C[AT][AT]GG
FauI 158 CCCGC CCCGC(4/6)
HincII 247 GTCGAC GT[CT]^[GA]AC
HindII 247 GTCGAC GT[CT]^[GA]AC
Ksp632I 748 CTCTTC CTCTTC(1/4)
MaeI 343 CTAG C^TAG
MflI 416 AGATCT [GA]^GATC[CT]
MluNI 713 TGGCCA TGG^CCA
MscI 713 TGGCCA TGG^CCA
PshAI 347 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 404 TCATGA T^CATGA
SalI 247 GTCGAC G^TCGAC
SexAI 251 ACCAGGT A^CC[AT]GGT
SfcI 901 CTACAG C^T[GA][CT]AG
SnaBI 367 TACGTA TAC^GTA
SspBI 767 TGTACA T^GTACA
StyI 342 CCTAGG C^C[AT][AT]GG
TspRI 291 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 416 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 177 GTAC GT^AC
768 GTAC
AvaI 512 CTCGGG C^[CT]CG[GA]G
642 CTCGGG
BanII 645 GGGCTC G[GA]GC[CT]^C
896 GGGCCC
BsaAI 367 TACGTA [CT]AC^GT[GA]
775 TACGTG
BsaJI 70 CCCTGG C^C[ACGT][ACGT]GG
342 CCTAGG
BseRI 619 CTCCTC GAGGAG(10/8)
723 CTCCTC
BsgI 388 CTGCAC GTGCAG(16/14)
564 CTGCAC
BsoBI 512 CTCGGG C^[CT]CG[GA]G
642 CTCGGG
BstXI 119 CCAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
236 CCAAAATGCTGG
Cfr13I 896 GGGCC G^G[ACGT]CC
897 GGCCC
DraII 895 GGGGCCC [GA]G^G[ACGT]CC[CT]
896 GGGCCCT
EcoO109I 895 GGGGCCC [GA]G^G[ACGT]CC[CT]
896 GGGCCCT
HaeIII 714 GGCC GG^CC
897 GGCC
HinfI 154 GACTC G^A[ACGT]TC
347 GACTC
PleI 154 GACTC GAGTC(4/5)
347 GACTC
RsaI 177 GTAC GT^AC
768 GTAC
Sau96I 896 GGGCC G^G[ACGT]CC
897 GGCCC
SfaNI 287 GATGC GCATC(5/9)
615 GATGC
TaqI 248 TCGA T^CGA
461 TCGA
Tsp509I 13 AATT ^AATT
660 AATT
TthHB8I 248 TCGA T^CGA
461 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AocI BbvI BmyI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Bsu36I Cac8I DdeI DpnII
Eco81I EcoRII Fnu4HI FokI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI TseI
Tsp45I