Restriction map for
pFN21AE5495
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HhaI HinP1I HindIII
KasI KpnI KspI MamI MluI
MroI MspA1I MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 459 CCGC CCGC(-3/-1)
AcsI 427 GAATTT [GA]^AATT[CT]
AcyI 250 GACGCC G[GA]^CG[CT]C
AlwNI 374 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 427 GAATTT [GA]^AATT[CT]
AspI 346 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 608 CCCGGG C^[CT]CG[GA]G
AvaII 345 GGACC G^G[AT]CC
BamHI 604 GGATCC G^GATCC
BbsI 922 GTCTTC GAAGAC(2/6)
BclI 731 TGATCA T^GATCA
BglI 442 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 368 AGATCT A^GATCT
BpmI 941 CTCCAG CTGGAG(16/14)
BpuAI 922 GTCTTC GAAGAC(2/6)
BsaHI 250 GACGCC G[GA]^CG[CT]C
BsoBI 608 CCCGGG C^[CT]CG[GA]G
BspHI 500 TCATGA T^CATGA
Cac8I 22 GCCAGC GC[ACGT]^[ACGT]GC
EcoNI 342 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 446 CCATGG C^C[AT][AT]GG
FbaI 731 TGATCA T^GATCA
HgaI 250 GACGC GACGC(5/10)
Hin1I 250 GACGCC G[GA]^CG[CT]C
HincII 1008 GTTAAC GT[CT]^[GA]AC
HindII 1008 GTTAAC GT[CT]^[GA]AC
HpaI 1008 GTTAAC GTT^AAC
MfeI 289 CAATTG C^AATTG
MunI 289 CAATTG C^AATTG
NcoI 446 CCATGG C^CATGG
NspI 872 ACATGC [GA]CATG^[CT]
PflFI 346 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 348 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 831 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 500 TCATGA T^CATGA
SexAI 347 ACCAGGT A^CC[AT]GGT
SfcI 963 CTATAG C^T[GA][CT]AG
SmaI 608 CCCGGG CCC^GGG
StuI 803 AGGCCT AGG^CCT
StyI 446 CCATGG C^C[AT][AT]GG
Tth111I 346 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 348 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 608 CCCGGG C^CCGGG
XmaI 608 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BanII 549 GGGCTC G[GA]GC[CT]^C
992 GGGCTC
BbvI 536 GCTGC GCAGC(8/12)
856 GCTGC
BcnI 608 CCCGG CC^[GC]GG
609 CCGGG
BfaI 415 CTAG C^TAG
439 CTAG
BsaAI 406 TACGTG [CT]AC^GT[GA]
463 TACGTG
BsmAI 294 GTCTC GTCTC(1/5)
518 GTCTC
BsmFI 57 GGGAC GGGAC(10/14)
141 GGGAC
BstYI 368 AGATCT [GA]^GATC[CT]
604 GGATCC
Cfr13I 345 GGACC G^G[ACGT]CC
792 GGGCC
Fnu4HI 536 GCTGC GC^[ACGT]GC
856 GCTGC
HapII 609 CCGG C^CGG
795 CCGG
HpaII 609 CCGG C^CGG
795 CCGG
ItaI 536 GCTGC GC^[ACGT]GC
856 GCTGC
MaeI 415 CTAG C^TAG
439 CTAG
MaeII 407 ACGT A^CGT
464 ACGT
MflI 368 AGATCT [GA]^GATC[CT]
604 GGATCC
MseI 958 TTAA T^TAA
1009 TTAA
MspI 609 CCGG C^CGG
795 CCGG
MwoI 330 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
442 GCCACCATGGC
NciI 608 CCCGG CC^[GC]GG
609 CCGGG
PleI 59 GACTC GAGTC(4/5)
143 GACTC
Sau96I 345 GGACC G^G[ACGT]CC
792 GGGCC
SfaNI 336 GATGC GCATC(5/9)
390 GATGC
TfiI 615 GATTC G^A[AT]TC
651 GAATC
Tru9I 958 TTAA T^TAA
1009 TTAA
TseI 536 GCTGC G^C[AT]GC
856 GCTGC
Tsp509I 290 AATT ^AATT
428 AATT
XcmI 74 CCAAACATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
332 CCAAGATGCTCCTGG
XhoII 368 AGATCT [GA]^GATC[CT]
604 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AlwI BalI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
DdeI DpnII DsaI EaeI EarI
EcoRII FokI HaeIII HinfI HphI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
Tsp45I TspRI