Restriction map for
pFN21AE5952
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# NO CUT ENZYMES ( 165 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BanI BbeI BbrPI
BbsI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 698 GACGTC GACGT^C
AcyI 698 GACGTC G[GA]^CG[CT]C
AseI 900 ATTAAT AT^TAAT
AsnI 900 ATTAAT AT^TAAT
Asp700I 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 95 TGGCCA TGG^CCA
BamHI 446 GGATCC G^GATCC
BanII 453 GGGCTC G[GA]GC[CT]^C
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BbvI 263 GCAGC GCAGC(8/12)
BciVI 153 GGATAC GTATCC(6/5)
BmyI 453 GGGCTC G[AGT]GC[ACT]^C
BsaHI 698 GACGTC G[GA]^CG[CT]C
BsgI 222 CTGCAC GTGCAG(16/14)
Bsp1286I 453 GGGCTC G[AGT]GC[ACT]^C
BspHI 404 TCATGA T^CATGA
BspMI 760 GCAGGT ACCTGC(4/8)
BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 283 GCTGGC GC[ACGT]^[ACGT]GC
EaeI 95 TGGCCA [CT]^GGCC[GA]
Fnu4HI 263 GCAGC GC^[ACGT]GC
Hin1I 698 GACGTC G[GA]^CG[CT]C
HindIII 707 AAGCTT A^AGCTT
HphI 729 TCACC GGTGA(8/7)
ItaI 263 GCAGC GC^[ACGT]GC
MaeII 699 ACGT A^CGT
MluNI 95 TGGCCA TGG^CCA
MscI 95 TGGCCA TGG^CCA
MseI 901 TTAA T^TAA
PaeR7I 656 CTCGAG C^TCGAG
PshBI 900 ATTAAT AT^TAAT
RcaI 404 TCATGA T^CATGA
SfaNI 475 GATGC GCATC(5/9)
SmlI 656 CTCGAG C^T[CT][GA]AG
SspI 579 AATATT AAT^ATT
TaqI 657 TCGA T^CGA
Tru9I 901 TTAA T^TAA
TseI 263 GCAGC G^C[AT]GC
TthHB8I 657 TCGA T^CGA
XcmI 98 CCATCTTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 656 CTCGAG C^TCGAG
XmnI 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
876 CCGC
AcsI 299 AAATTT [GA]^AATT[CT]
416 AAATTT
AluI 161 AGCT AG^CT
708 AGCT
ApoI 299 AAATTT [GA]^AATT[CT]
416 AAATTT
AvaI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
AvaII 90 GGTCC G^G[AT]CC
207 GGACC
BpmI 689 CTGGAG CTGGAG(16/14)
929 CTGGAG
BsaI 408 GAGACC GGTCTC(1/5)
509 GGTCTC
BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG
450 CCTGGG
BsmAI 408 GAGAC GTCTC(1/5)
510 GTCTC
BsmFI 696 GGGAC GGGAC(10/14)
198 GTCCC
BsmI 331 GAATGC GAATGC(1/-1)
776 GCATTC
BsoBI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
BsrDI 317 CATTGC GCAATG(2/0)
566 GCAATG
BstYI 446 GGATCC [GA]^GATC[CT]
685 GGATCT
Cfr13I 90 GGTCC G^G[ACGT]CC
207 GGACC
DpnII 447 GATC ^GATC
686 GATC
EarI 79 CTCTTC CTCTTC(1/4)
586 GAAGAG
Ksp632I 79 CTCTTC CTCTTC(1/4)
586 GAAGAG
MboI 447 GATC ^GATC
686 GATC
MflI 446 GGATCC [GA]^GATC[CT]
685 GGATCT
MwoI 283 GCTGGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
326 GCTCCGAATGC
NdeII 447 GATC ^GATC
686 GATC
NlaIV 325 GGCTCC GG[ACGT]^[ACGT]CC
446 GGATCC
Sau3AI 447 GATC ^GATC
686 GATC
Sau96I 90 GGTCC G^G[ACGT]CC
207 GGACC
SfcI 458 CTACAG C^T[GA][CT]AG
479 CTGTAG
Tsp509I 300 AATT ^AATT
417 AATT
XhoII 446 GGATCC [GA]^GATC[CT]
685 GGATCT
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI AlwI BseRI BsiYI BslI
BssKI BstNI DdeI EcoRII HaeIII
HinfI MaeIII MboII MnlI MslI
MvaI NlaIII RsaI ScrFI TfiI
Tsp45I TspRI