Restriction map for
pFN21AE5493
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AflIII 71 ACACGT A^C[GA][CT]GT
Asp700I 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 446 GGATCC G^GATCC
BanII 453 GGGCTC G[GA]GC[CT]^C
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BclI 792 TGATCA T^GATCA
BglII 908 AGATCT A^GATCT
BmyI 453 GGGCTC G[AGT]GC[ACT]^C
BpmI 689 CTGGAG CTGGAG(16/14)
BsaI 509 GGTCTC GGTCTC(1/5)
BsgI 222 CTGCAC GTGCAG(16/14)
BsmAI 510 GTCTC GTCTC(1/5)
BsmFI 198 GTCCC GGGAC(10/14)
BsmI 898 GCATTC GAATGC(1/-1)
Bsp1286I 453 GGGCTC G[AGT]GC[ACT]^C
BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI 538 GAATTC G^AATTC
EcoT14I 42 CCTTGG C^C[AT][AT]GG
FbaI 792 TGATCA T^GATCA
HindIII 707 AAGCTT A^AGCTT
MaeII 73 ACGT A^CGT
NlaIV 446 GGATCC GG[ACGT]^[ACGT]CC
PaeR7I 656 CTCGAG C^TCGAG
SmlI 656 CTCGAG C^T[CT][GA]AG
SspI 579 AATATT AAT^ATT
StyI 42 CCTTGG C^C[AT][AT]GG
TaqI 657 TCGA T^CGA
TspRI 317 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 657 TCGA T^CGA
XhoI 656 CTCGAG C^TCGAG
XmnI 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
876 CCGC
AvaI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
AvaII 90 GGTCC G^G[AT]CC
207 GGACC
BbvI 263 GCAGC GCAGC(8/12)
760 GCAGC
BfaI 893 CTAG C^TAG
1018 CTAG
BseRI 139 CTCCTC GAGGAG(10/8)
184 CTCCTC
BsoBI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
Bsp1407I 773 TGTACA T^GTACA
1031 TGTACA
BspHI 404 TCATGA T^CATGA
902 TCATGA
BsrGI 773 TGTACA T^GTACA
1031 TGTACA
BstF5I 36 CATCC GGATG(2/0)
837 CATCC
EarI 79 CTCTTC CTCTTC(1/4)
586 GAAGAG
Fnu4HI 263 GCAGC GC^[ACGT]GC
760 GCAGC
FokI 36 CATCC GGATG(9/13)
837 CATCC
HphI 888 GGTGA GGTGA(8/7)
729 TCACC
ItaI 263 GCAGC GC^[ACGT]GC
760 GCAGC
Ksp632I 79 CTCTTC CTCTTC(1/4)
586 GAAGAG
MaeI 893 CTAG C^TAG
1018 CTAG
MwoI 346 GCTGTTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
646 GCTTCCTATGC
RcaI 404 TCATGA T^CATGA
902 TCATGA
SfaNI 475 GATGC GCATC(5/9)
98 GCATC
SspBI 773 TGTACA T^GTACA
1031 TGTACA
TseI 263 GCAGC G^C[AT]GC
760 GCAGC
Tsp45I 292 GTCAC ^GT[GC]AC
889 GTGAC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BsaJI BsiYI BslI BsrDI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EcoRII HaeIII HinfI MaeIII MboI
MboII MflI MnlI MslI MvaI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfcI TfiI Tsp509I XhoII