Restriction map for
pFN21AE5969
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BspDI BspEI BspLU11I
BsrBI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI Tru9I Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AspHI 896 GAGCAC G[AT]GC[AT]^C
BalI 95 TGGCCA TGG^CCA
BamHI 446 GGATCC G^GATCC
BanII 453 GGGCTC G[GA]GC[CT]^C
BbvI 263 GCAGC GCAGC(8/12)
BpmI 689 CTGGAG CTGGAG(16/14)
BsiHKAI 896 GAGCAC G[AT]GC[AT]^C
BsmI 331 GAATGC GAATGC(1/-1)
Bsp1407I 773 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BspMI 760 GCAGGT ACCTGC(4/8)
BsrDI 566 GCAATG GCAATG(2/0)
BsrGI 773 TGTACA T^GTACA
BstEII 888 GGTGACC G^GT[ACGT]ACC
BstPI 888 GGTGACC G^GT[ACGT]ACC
BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 95 TGGCCA [CT]^GGCC[GA]
EarI 79 CTCTTC CTCTTC(1/4)
EcoO65I 888 GGTGACC G^GT[ACGT]ACC
Fnu4HI 263 GCAGC GC^[ACGT]GC
HincII 191 GTCAAC GT[CT]^[GA]AC
HindII 191 GTCAAC GT[CT]^[GA]AC
HindIII 707 AAGCTT A^AGCTT
ItaI 263 GCAGC GC^[ACGT]GC
Ksp632I 79 CTCTTC CTCTTC(1/4)
MluNI 95 TGGCCA TGG^CCA
MscI 95 TGGCCA TGG^CCA
PaeR7I 656 CTCGAG C^TCGAG
RcaI 404 TCATGA T^CATGA
SmlI 656 CTCGAG C^T[CT][GA]AG
SspBI 773 TGTACA T^GTACA
SspI 579 AATATT AAT^ATT
TaqI 657 TCGA T^CGA
TseI 263 GCAGC G^C[AT]GC
TthHB8I 657 TCGA T^CGA
XcmI 98 CCATCTTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 656 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
876 CCGC
AcsI 299 AAATTT [GA]^AATT[CT]
416 AAATTT
ApoI 299 AAATTT [GA]^AATT[CT]
416 AAATTT
Asp700I 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
586 GAAAAGGTTC
AvaI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
AvaII 90 GGTCC G^G[AT]CC
207 GGACC
BmyI 453 GGGCTC G[AGT]GC[ACT]^C
896 GAGCAC
BsaI 408 GAGACC GGTCTC(1/5)
509 GGTCTC
BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG
450 CCTGGG
BseRI 139 CTCCTC GAGGAG(10/8)
184 CTCCTC
BsmAI 408 GAGAC GTCTC(1/5)
510 GTCTC
BsoBI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
Bsp1286I 453 GGGCTC G[AGT]GC[ACT]^C
896 GAGCAC
BstYI 446 GGATCC [GA]^GATC[CT]
685 GGATCT
HphI 888 GGTGA GGTGA(8/7)
729 TCACC
MaeIII 536 GTGAC ^GT[ACGT]AC
889 GTGAC
MflI 446 GGATCC [GA]^GATC[CT]
685 GGATCT
NlaIV 325 GGCTCC GG[ACGT]^[ACGT]CC
446 GGATCC
SfaNI 463 GATGC GCATC(5/9)
475 GATGC
SfcI 479 CTGTAG C^T[GA][CT]AG
867 CTACAG
Tsp45I 536 GTGAC ^GT[GC]AC
889 GTGAC
Tsp509I 300 AATT ^AATT
417 AATT
TspRI 107 CAGTG CA[GC]TG[ACGT][ACGT]^
317 CACTG
XhoII 446 GGATCC [GA]^GATC[CT]
685 GGATCT
XmnI 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
586 GAAAAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI AlwI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII FokI HaeIII HinfI
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI TfiI