Restriction map for
pFN21AE5968
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 399 CCGC CCGC(-3/-1)
AfaI 484 GTAC GT^AC
AflII 232 CTTAAG C^TTAAG
AluI 191 AGCT AG^CT
AocI 870 CCTGAGG CC^T[ACGT]AGG
ApaI 893 GGGCCC GGGCC^C
AspHI 467 GTGCTC G[AT]GC[AT]^C
BanII 893 GGGCCC G[GA]GC[CT]^C
BbsI 823 GTCTTC GAAGAC(2/6)
BbvI 719 GCAGC GCAGC(8/12)
BcnI 654 CCGGG CC^[GC]GG
BfrI 232 CTTAAG C^TTAAG
BpuAI 823 GTCTTC GAAGAC(2/6)
BsgI 323 GTGCAG GTGCAG(16/14)
BsiHKAI 467 GTGCTC G[AT]GC[AT]^C
BsmFI 538 GTCCC GGGAC(10/14)
BspMI 715 ACCTGC ACCTGC(4/8)
BstYI 784 AGATCC [GA]^GATC[CT]
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
Cac8I 559 GCCTGC GC[ACGT]^[ACGT]GC
DraIII 279 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 568 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 651 TGGCCG [CT]^GGCC[GA]
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
Fnu4HI 719 GCAGC GC^[ACGT]GC
HapII 654 CCGG C^CGG
HpaII 654 CCGG C^CGG
ItaI 719 GCAGC GC^[ACGT]GC
MaeII 777 ACGT A^CGT
MflI 784 AGATCC [GA]^GATC[CT]
MseI 233 TTAA T^TAA
MspI 654 CCGG C^CGG
MwoI 343 GCATCTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 654 CCGGG CC^[GC]GG
RsaI 484 GTAC GT^AC
SmlI 232 CTTAAG C^T[CT][GA]AG
TaqI 454 TCGA T^CGA
Tru9I 233 TTAA T^TAA
TseI 719 GCAGC G^C[AT]GC
Tsp45I 550 GTGAC ^GT[GC]AC
TthHB8I 454 TCGA T^CGA
XhoII 784 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 670 GTCTAC GT^[AC][GT]AC
763 GTCTAC
BsaJI 509 CCAGGG C^C[ACGT][ACGT]GG
572 CCTGGG
BsrI 115 ACTGG ACTGG(1/-1)
280 ACTGG
BssSI 902 CACGAG CACGAG(-5/-1)
616 CTCGTG
BstF5I 81 CATCC GGATG(2/0)
396 CATCC
BstNI 509 CCAGG CC^[AT]GG
572 CCTGG
DdeI 184 CTTAG C^T[ACGT]AG
871 CTGAG
DraII 892 GGGGCCC [GA]G^G[ACGT]CC[CT]
893 GGGCCCT
EarI 76 CTCTTC CTCTTC(1/4)
383 CTCTTC
EcoO109I 892 GGGGCCC [GA]G^G[ACGT]CC[CT]
893 GGGCCCT
EcoRII 509 CCAGG ^CC[AT]GG
572 CCTGG
FokI 81 CATCC GGATG(9/13)
396 CATCC
HphI 549 GGTGA GGTGA(8/7)
885 GGTGA
Ksp632I 76 CTCTTC CTCTTC(1/4)
383 CTCTTC
MvaI 509 CCAGG CC^[AT]GG
572 CCTGG
NlaIV 892 GGGGCC GG[ACGT]^[ACGT]CC
893 GGGCCC
PleI 552 GACTC GAGTC(4/5)
909 GAGTC
PstI 686 CTGCAG CTGCA^G
717 CTGCAG
SfaNI 241 GCATC GCATC(5/9)
343 GCATC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AlwI BmyI BsiYI BslI Bsp1286I
BsrDI BssKI Cfr13I DpnII HaeIII
HinfI MaeIII MboI MboII MnlI
MslI NdeII NlaIII Sau3AI Sau96I
ScrFI SfcI TfiI Tsp509I TspRI