Restriction map for
pFN21AE5484
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssKI
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 633 GTATAC GT^[AC][GT]AC
AciI 862 GCGG CCGC(-3/-1)
AlwI 180 GATCC GGATC(4/5)
Asp700I 376 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 729 GAGCTC G[AT]GC[AT]^C
BanII 729 GAGCTC G[GA]GC[CT]^C
BbvI 467 GCTGC GCAGC(8/12)
BfaI 679 CTAG C^TAG
BglI 682 GCCATTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 554 GGTCTC GGTCTC(1/5)
BseRI 948 GAGGAG GAGGAG(10/8)
BsiHKAI 729 GAGCTC G[AT]GC[AT]^C
BsmFI 70 GGGAC GGGAC(10/14)
Bsp1407I 260 TGTACA T^GTACA
BsrDI 135 CATTGC GCAATG(2/0)
BsrGI 260 TGTACA T^GTACA
BssSI 701 CTCGTG CACGAG(-5/-1)
Bst1107I 633 GTATAC GTA^TAC
BstZ17I 633 GTATAC GTA^TAC
Cac8I 787 GCAAGC GC[ACGT]^[ACGT]GC
EarI 119 CTCTTC CTCTTC(1/4)
EcoNI 818 CCTACACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 467 GCTGC GC^[ACGT]GC
HincII 250 GTTGAC GT[CT]^[GA]AC
HindII 250 GTTGAC GT[CT]^[GA]AC
HphI 426 TCACC GGTGA(8/7)
ItaI 467 GCTGC GC^[ACGT]GC
Ksp632I 119 CTCTTC CTCTTC(1/4)
MaeI 679 CTAG C^TAG
MaeII 346 ACGT A^CGT
MseI 566 TTAA T^TAA
NspI 481 GCATGT [GA]CATG^[CT]
PleI 206 GACTC GAGTC(4/5)
SacI 729 GAGCTC GAGCT^C
SspBI 260 TGTACA T^GTACA
TaqI 192 TCGA T^CGA
TfiI 339 GATTC G^A[AT]TC
Tru9I 566 TTAA T^TAA
TseI 467 GCTGC G^C[AT]GC
TthHB8I 192 TCGA T^CGA
XcmI 317 CCATTAGATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 376 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 222 GTAC GT^AC
261 GTAC
BglII 461 AGATCT A^GATCT
994 AGATCT
BmyI 277 GTGCCC G[AGT]GC[ACT]^C
729 GAGCTC
BsaJI 363 CCTTGG C^C[ACGT][ACGT]GG
387 CCTTGG
BsmAI 951 GAGAC GTCTC(1/5)
555 GTCTC
Bsp1286I 277 GTGCCC G[AGT]GC[ACT]^C
729 GAGCTC
BsrI 494 CCAGT ACTGG(1/-1)
624 CCAGT
BstYI 461 AGATCT [GA]^GATC[CT]
994 AGATCT
Cfr13I 360 GGCCC G^G[ACGT]CC
491 GGGCC
EcoT14I 363 CCTTGG C^C[AT][AT]GG
387 CCTTGG
HinfI 206 GACTC G^A[ACGT]TC
339 GATTC
MboII 459 GAAGA GAAGA(8/7)
120 TCTTC
MflI 461 AGATCT [GA]^GATC[CT]
994 AGATCT
RsaI 222 GTAC GT^AC
261 GTAC
Sau96I 360 GGCCC G^G[ACGT]CC
491 GGGCC
StyI 363 CCTTGG C^C[AT][AT]GG
387 CCTTGG
XhoII 461 AGATCT [GA]^GATC[CT]
994 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BsiYI BslI DdeI DpnII
HaeIII MaeIII MboI MnlI MslI
MwoI NdeII NlaIII Sau3AI SfcI
Tsp45I TspRI