Restriction map for
pFN21AE6010
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 99 CGCG CG^CG
AccIII 554 TCCGGA T^CCGGA
AcsI 680 AAATTC [GA]^AATT[CT]
AflIII 770 ACATGT A^C[GA][CT]GT
AgeI 362 ACCGGT A^CCGGT
AlwNI 414 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 680 AAATTC [GA]^AATT[CT]
Asp700I 705 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 74 GGATCC G^GATCC
BclI 401 TGATCA T^GATCA
BfaI 931 CTAG C^TAG
BpmI 67 CTGGAG CTGGAG(16/14)
BsaI 741 GGTCTC GGTCTC(1/5)
BseAI 554 TCCGGA T^CCGGA
BseRI 850 CTCCTC GAGGAG(10/8)
BsgI 388 CTGCAC GTGCAG(16/14)
Bsp1407I 773 TGTACA T^GTACA
BspEI 554 TCCGGA T^CCGGA
BspHI 404 TCATGA T^CATGA
BspLU11I 770 ACATGT A^CATGT
BspMI 718 ACCTGC ACCTGC(4/8)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BsrGI 773 TGTACA T^GTACA
BssSI 514 CACGAG CACGAG(-5/-1)
BstUI 99 CGCG CG^CG
BstYI 74 GGATCC [GA]^GATC[CT]
CfoI 492 GCGC GCG^C
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
DraIII 514 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 586 GACGGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 401 TGATCA T^GATCA
HhaI 492 GCGC GCG^C
HinP1I 492 GCGC G^CGC
HinfI 47 GATTC G^A[ACGT]TC
MaeI 931 CTAG C^TAG
MflI 74 GGATCC [GA]^GATC[CT]
MroI 554 TCCGGA T^CCGGA
MseI 254 TTAA T^TAA
MvnI 99 CGCG CG^CG
PflMI 513 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 362 ACCGGT A^CCGGT
PvuII 238 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SspBI 773 TGTACA T^GTACA
StuI 707 AGGCCT AGG^CCT
TaqI 584 TCGA T^CGA
TfiI 47 GATTC G^A[AT]TC
Tru9I 254 TTAA T^TAA
Tsp509I 681 AATT ^AATT
TthHB8I 584 TCGA T^CGA
Van91I 513 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 74 GGATCC [GA]^GATC[CT]
XmnI 705 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BcnI 146 CCCGG CC^[GC]GG
423 CCGGG
BmyI 508 GGGCAC G[AGT]GC[ACT]^C
896 GGGCAC
BsaWI 362 ACCGGT [AT]^CCGG[AT]
554 TCCGGA
BsmAI 477 GTCTC GTCTC(1/5)
742 GTCTC
BsmFI 144 GTCCC GGGAC(10/14)
624 GTCCC
Bsp1286I 508 GGGCAC G[AGT]GC[ACT]^C
896 GGGCAC
BstXI 131 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
461 CCAACTCCTTGG
DdeI 211 CTCAG C^T[ACGT]AG
874 CTCAG
EcoT14I 467 CCTTGG C^C[AT][AT]GG
629 CCTTGG
FauI 500 CCCGC CCCGC(4/6)
544 CCCGC
MspA1I 238 CAGCTG C[AC]G^C[GT]G
501 CCGCTG
NciI 146 CCCGG CC^[GC]GG
423 CCGGG
NspI 770 ACATGT [GA]CATG^[CT]
776 ACATGC
SapI 300 GCTCTTC GCTCTTC(1/4)
825 GCTCTTC
StyI 467 CCTTGG C^C[AT][AT]GG
629 CCTTGG
XcmI 128 CCACCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
272 CCATCAGCTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanI BbvI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EaeI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI