Restriction map for
pFN21AE5388
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 216 GTATAC GT^[AC][GT]AC
AocI 675 CCTCAGG CC^T[ACGT]AGG
AvaII 758 GGTCC G^G[AT]CC
BamHI 680 GGATCC G^GATCC
BanII 438 GGGCTC G[GA]GC[CT]^C
BbrPI 717 CACGTG CAC^GTG
BclI 809 TGATCA T^GATCA
BsaAI 717 CACGTG [CT]AC^GT[GA]
BsaBI 99 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 723 CTCCTC GAGGAG(10/8)
BspHI 404 TCATGA T^CATGA
Bst1107I 216 GTATAC GTA^TAC
BstZ17I 216 GTATAC GTA^TAC
Bsu36I 675 CCTCAGG CC^T[ACGT]AGG
CfoI 424 GCGC GCG^C
DraI 852 TTTAAA TTT^AAA
EarI 65 CTCTTC CTCTTC(1/4)
Eco81I 675 CCTCAGG CC^T[ACGT]AGG
FbaI 809 TGATCA T^GATCA
HapII 699 CCGG C^CGG
HhaI 424 GCGC GCG^C
HinP1I 424 GCGC G^CGC
HpaII 699 CCGG C^CGG
Ksp632I 65 CTCTTC CTCTTC(1/4)
MamI 99 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 853 TTAA T^TAA
MspI 699 CCGG C^CGG
NcoI 350 CCATGG C^CATGG
PmaCI 717 CACGTG CAC^GTG
PmlI 717 CACGTG CAC^GTG
PshAI 361 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 427 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
ScaI 332 AGTACT AGT^ACT
Tru9I 853 TTAA T^TAA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
780 CCGC
BalI 245 TGGCCA TGG^CCA
461 TGGCCA
BpmI 845 CTCCAG CTGGAG(16/14)
205 CTGGAG
BsgI 324 CTGCAC GTGCAG(16/14)
564 CTGCAC
BsiYI 671 CCATCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
692 CCAGTGGCCGG
BslI 671 CCATCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
692 CCAGTGGCCGG
BsmAI 895 GAGAC GTCTC(1/5)
646 GTCTC
Bsp1407I 101 TGTACA T^GTACA
656 TGTACA
BsrDI 465 CATTGC GCAATG(2/0)
540 CATTGC
BsrGI 101 TGTACA T^GTACA
656 TGTACA
BstXI 236 CCAAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
248 CCATCCTACTGG
BstYI 680 GGATCC [GA]^GATC[CT]
804 GGATCT
DsaI 350 CCATGG C^C[GA][CT]GG
737 CCGTGG
EcoT14I 350 CCATGG C^C[AT][AT]GG
800 CCTTGG
HinfI 843 GACTC G^A[ACGT]TC
897 GACTC
MaeIII 536 GTGAC ^GT[ACGT]AC
841 GTGAC
MflI 680 GGATCC [GA]^GATC[CT]
804 GGATCT
MluNI 245 TGGCCA TGG^CCA
461 TGGCCA
MscI 245 TGGCCA TGG^CCA
461 TGGCCA
MspA1I 363 CCGCTG C[AC]G^C[GT]G
427 CAGCTG
PleI 843 GACTC GAGTC(4/5)
897 GACTC
SfaNI 796 GATGC GCATC(5/9)
729 GCATC
SspBI 101 TGTACA T^GTACA
656 TGTACA
StyI 350 CCATGG C^C[AT][AT]GG
800 CCTTGG
Tsp45I 536 GTGAC ^GT[GC]AC
841 GTGAC
XhoII 680 GGATCC [GA]^GATC[CT]
804 GGATCT
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AlwI AspHI BbvI
BmyI BsaJI BsiHKAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeII
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI TspRI