Restriction map for
pFN21AE2559
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1286I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeIII Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MboII MfeI MluI
MluNI MscI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tru9I Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 218 TCCGGA T^CCGGA
AfaI 345 GTAC GT^AC
Aor51HI 125 AGCGCT AGC^GCT
BbvCI 207 CCTCAGC CCTCAGC(-5/-2)
BglII 308 AGATCT A^GATCT
BpmI 382 CTGGAG CTGGAG(16/14)
BsaWI 218 TCCGGA [AT]^CCGG[AT]
BseAI 218 TCCGGA T^CCGGA
BsmBI 139 GAGACG CGTCTC(1/5)
BsmFI 178 GTCCC GGGAC(10/14)
Bsp1407I 344 TGTACA T^GTACA
BspEI 218 TCCGGA T^CCGGA
BsrGI 344 TGTACA T^GTACA
BsrI 201 CCAGT ACTGG(1/-1)
BstXI 167 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 308 AGATCT [GA]^GATC[CT]
DpnII 309 GATC ^GATC
Eco47III 125 AGCGCT AGC^GCT
EcoT14I 299 CCATGG C^C[AT][AT]GG
HaeII 125 AGCGCT [GA]GCGC^[CT]
HgaI 26 GCGTC GACGC(5/10)
HindIII 51 AAGCTT A^AGCTT
HphI 264 GGTGA GGTGA(8/7)
MaeII 142 ACGT A^CGT
MboI 309 GATC ^GATC
MflI 308 AGATCT [GA]^GATC[CT]
MroI 218 TCCGGA T^CCGGA
MspA1I 154 CCGCTG C[AC]G^C[GT]G
NcoI 299 CCATGG C^CATGG
NdeII 309 GATC ^GATC
PleI 385 GAGTC GAGTC(4/5)
RsaI 345 GTAC GT^AC
Sau3AI 309 GATC ^GATC
SfaNI 212 GCATC GCATC(5/9)
SfcI 312 CTACAG C^T[GA][CT]AG
SmlI 415 CTTGAG C^T[CT][GA]AG
SspBI 344 TGTACA T^GTACA
StyI 299 CCATGG C^C[AT][AT]GG
TfiI 83 GAATC G^A[AT]TC
XcmI 363 CCAGGAGACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 308 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 52 AGCT AG^CT
188 AGCT
AlwNI 184 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
423 CAGACCCTG
AvaII 177 GGTCC G^G[AT]CC
231 GGTCC
BcnI 87 CCGGG CC^[GC]GG
111 CCCGG
BsiYI 397 CCCAGGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
398 CCAGGGATGGG
BslI 397 CCCAGGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
398 CCAGGGATGGG
CfoI 126 GCGC GCG^C
293 GCGC
Cfr13I 177 GGTCC G^G[ACGT]CC
231 GGTCC
DdeI 208 CTCAG C^T[ACGT]AG
235 CTGAG
DsaI 242 CCACGG C^C[GA][CT]GG
299 CCATGG
FauI 153 CCCGC CCCGC(4/6)
180 CCCGC
HhaI 126 GCGC GCG^C
293 GCGC
HinP1I 126 GCGC G^CGC
293 GCGC
HinfI 83 GAATC G^A[ACGT]TC
385 GAGTC
NciI 87 CCGGG CC^[GC]GG
111 CCCGG
NlaIV 177 GGTCCC GG[ACGT]^[ACGT]CC
221 GGAGCC
Sau96I 177 GGTCC G^G[ACGT]CC
231 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI BbvI BsaJI BsmAI BssKI
BstF5I BstNI EcoRII Fnu4HI FokI
HapII HpaII ItaI MnlI MspI
MvaI MwoI NlaIII ScrFI TseI